Very Fast CRISPR on Demanddoi:10.1016/j.bpj.2019.11.337Yang LiuRoger ZouYuta NihongakiShuaixin HeShiva RazaviBin WuTaekjip HaBiophysical Journal
CRISPR/Cas (Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR associated nucleases) is a powerful component of the prokaryotic immune system that has been adapted for targeted genetic engineering in higher organisms. A key element of CRISPR/Cas is the "guide" RNA (gRNA) that is ~20...
(University of Greifswald, Greifswald, Germany) for kindly sharing the fas1- DfasD2 strain, Alexandra Bergman for help with constructing the ELO3 deletion cassette, Raphael Ferreira for help with constructing the CRISPR/Cas9 plasmid, Tyler Doughty for help with final polishing of the manuscript, ...
Pliatsika, V. & Rigoutsos, I. "Off-Spotter": very fast and exhaustive enumeration of genomic lookalikes for designing CRISPR/Cas guide RNAs. Biol. Direct 10, 4 (2015).Pliatsika, V. and I. Rigoutsos (2015). ""Off-Spotter": very fast and exhaustive enumeration of genomic lookalikes...
"Off-Spotter": Very fast and exhaustive enumeration of genomic lookalikes for designing CRISPR/Cas guide RNAs. Biol. Direct 2015, 10, 4. [CrossRef]Off-Spotter":very fast and exhaustive enumeration of genomic lookalikes for designing CRISPR/Cas guide RNAs. Pliatsika V,Rigoutsos I. Biol ...
CRISPR/Cas9 technology34,35and modular pathway engineering36for yeast genetic engineering, we could easily transfer our metabolic engineering strategy to different genetic background strains. Here we mainly focused on integrating the genes into chromosome instead of using episomal expression to get more ...