CRISPRdirect enables users to easily select rational target sequences with minimized off-target sites by performing exhaustive searches against genomic sequences. The server currently incorporates the genomic sequences of human, mouse, rat, marmoset, pig, chicken, frog, zebrafish, Ciona, fruit fly, ...
Therefore, CRISPR tiling screens enable the identification of novel drug resistance mutations located farther away from the direct drug binding pocket. Although CRISPR-directed evolution using DNA DSBs is very feasible, other CRISPR systems are available to perform targeted mutagenesis at endogenous loci ...
CRISPR controversydoi:10.1016/S0262-4079(17)31345-3SDOSNew Scientist
因此，即使Tetraloop和Loop2被其他aptamer占用（如SAM CRISPR激活系统等），也不影响捕获序列的使用。文章同时提供了Direct-seq兼容10x RNA-seq 3’ kit，10x RNA-seq 5’ kit和Fluidigm C1/SMART-seq的参考实验流程。据文章报道，在一个使用了10x RNA-seq 3’ v3试剂盒的测试中，由于作者在文库构建过程中使用了...
Nucleic acid detection with CRISPR-Cas13a/C2c2 We combine the collateral effect of Cas13a with isothermal amplification to establish a CRISPR-based diagnostic (CRISPR-Dx), providing rapid DNA or RNA detection with attomolar sensitivity and single-base mismatch specificity. We use this ... JS Goot...
CRISPR/Cas-mediated genome editing in the rat via direct injection of one-cell embryos. Liu M, Chen Y, Wu L, Li Y, Ma X, Liu M et al (2014) CRISPR/Cas-mediated genome editing in the rat via direct injection of one-cell embryos... Y Shao,Y Guan,L Wang,... - 《Nature ...
西湖大学近期在开放获取期刊Genome Biology上发表了一项研究成果Direct-seq,该研究开发了一种将CRISPR遗传筛选与单细胞RNA-seq结合的新技术,通过改造gRNA序列,在单细胞水平将细胞的“基因型-基因表达谱-表型”关联起来,从而克服了上述两点局限性。...
Jeon, Y., Choi, Y.H., Jang, Y.et al.Direct observation of DNA target searching and cleavage by CRISPR-Cas12a.Nat Commun9, 2777 (2018). https://doi.org/10.1038/s41467-018-05245-x Download citation Received08 March 2018 Accepted20 June 2018 ...
Conventional CRISPR–Cas systems maintain genomic integrity by leveraging guide RNAs for the nuclease-dependent degradation of mobile genetic elements, including plasmids and viruses. Here we describe a notable inversion of this paradigm, in which bacterial Tn7-like transposons have co-opted nuclease-def...
A Robust CRISPR/Cas9 System for Convenient, High-Efficiency Multiplex Genome Editing in Monocot and Dicot Plants - ScienceDirect CRISPR/Cas9 染色体指向系统被用于许多种类.然而,为植物报导的 mostCRISPR/Cas9 系统能仅仅修改一个或一些指向地点.这里,我们报导一个柔韧的 CRISPR/Cas9 向量系统,利... X Ma,Q...