The vacuolar H+-ATPase (V-ATPase) is an ATP-dependent proton pump that is essential for cellular homeostasis. V-ATPase activity is controlled by the regulated assembly of the enzyme from its component V1 and V0 domains. We previously reported that amino acid starvation rapidly increases V-...
v-ATPase enzyme activity assay. Freshly isolated lysosomal fractions were used to measure the v-ATPase activity by an ATP/NADPH-coupled assay61. Briefly 20–50 mg of lysosomal protein was thoroughly mixed and incubated in 1 ml of reaction buffer containing 25 mM TEA (triethanolamine) pH 7.4, ...
1、酶联板(Assay plate ):一块(96孔或者48孔)。 2、标准品(Standard):2瓶(冻干品)。 3、样品稀释液(Sample Diluent):1×20ml/瓶。 4、生物素标记抗体稀释液(Biotin-antibody Diluent):1×10ml/瓶。 5、辣根过氧化物酶标记亲和素稀释液 (HRP-avidin Diluent):1×10ml/瓶。
ATPase activity竹嵌紋病毒(Bamboo mosaic virus,BaMV)為單股正意的RNA病毒,基因體包含五個轉譯架構(open reading frames,ORFs),ORF1可轉譯出分子量為155 kDa的複製酵素(replicase),經由胺基酸比對,中段區域含有多個特定保留性motifs,推測為類解旋酵素,並屬於病毒RNA解旋酵素superfamily 1(SF 1)的一員.先前的研究...
V-ATPase activity is essential for post-implantation development11, and no disease-causing mutations in these isoforms have been identified. However, loss-of-function mutations MGH Center for Systems Biology, Program in Membrane Biology & Division of Nephrology, Richard B. Simches Research Center, ...
We reported previously that SNX10 is a unique member of the SNX family genes in that it has vacuolation activity in cells. We investigate the biological function of SNX10 by loss-of-function assay in this study and demonstrate that SNX10 is required for the formation of primary cilia in ...
D730E, Q733A, Q733K, Q733R, G827A and G827V were unstable in the ATPase activity assay. In conclusion, the mutations at motif V of helicase-like domain of BaMV enhanced affinity to ATP but decreased catalytic efficiency. 展开
Assay-dependent - 产品规格 种属反应 Human 宿主/亚型 Rabbit / IgG 分类 Polyclonal 类型 Antibody 抗原 Recombinant Human V-type proton ATPase subunit G 1 protein (2-118AA) 查看免疫原 偶联物 Unconjugated 形式 Liquid 浓度 3 mg/mL 纯化类型 Protein G 保存液 PBS, pH...
ATPase activity assay. ATPase activity assays measure released phosphate based on the formation of coloured complex between malachite green reagent and free phosphate according to the manufacturer's instructions using the Quantichro- meTM ATPase/GTPase Assay Kit (BioAssay Systems). Briefly, 1 mM of ...
52 Recent reports also highlight the collective roles of autophagy and TGF-β in bovine mammary epithelial cells.53 V-ATPase is crucial for the integrity of both endolysosomal and autophagic vacuoles and disruption of V-ATPase activity has distinct and context- dependent effects on signaling by ...