研究结论：USP7与PD-L1相互作用并使PD-L1去泛素化，从而促进PD-L1的稳定性。4.阻断USP7使癌细胞对T细胞杀伤力敏感 阻断USP7诱导的PD-L1的表达量减少是否会影响PD-L1和PD-1之间的结合以及肿瘤细胞对T细胞介导的杀伤的敏感性仍不清楚。MGC-803细胞分别用USP7 sgRNA-慢病毒或Almac4处理，然后用重组蛋白可视化膜上...
为了进一步验证USP7可以对PD-L1进行去泛素化,在有无HA-USP7的情况下,将Flag-PD-L1与HA-His-Ub共同转染到HEK293细胞中。在有MG132但没有HA-USP7的情况下,PD-L1被强烈泛素化,而HA-USP7消除了PD-L1泛素化。为了测试内源性PD-L1是否也受到USP7的类似调控,在MGC-803细胞中通过基因敲除了USP7,结果表明内源...
为了进一步验证USP7可以对PD-L1进行去泛素化,在有无HA-USP7的情况下,将Flag-PD-L1与HA-His-Ub共同转染到HEK293细胞中。在有MG132但没有HA-USP7的情况下,PD-L1被强烈泛素化,而HA-USP7消除了PD-L1泛素化。为了测试内源性PD-L1是否也受到USP7的类似调控,在MGC-803细胞中通过基因敲除了USP7,结果表明内源...
这说明 USP7 抑制促进了 DCs 的成熟,并改变了细胞因子的产生模式,使其向促炎方向转变。 USP7 抑制对 PD-L1 和 PD-L2 表达的影响:研究人员再次运用 FACS 技术检测发现,USP7 抑制显著降低了 NOD.Stat5b-CA 小鼠和 NOD 小鼠 DCs 中 PD-L1 和 PD-L2 的表达。由于 PD-L1 和 PD-L2 在诱导外周免疫耐受中...
PD-L1: Programmed Cell Death Ligand 1 CTLA-4: Cytotoxic T-lymphocyte Associated protein 4 BC: Breast Cancer UALCAN: The University of ALabama at Birmingham CANcer References Grinda T, Antoine A, Jacot W, Blaye C, Cottu P-H, Diéras V, et al. Evolution of overall survival and rece...
USP7 inhibitor can also downregulate PD-L1 expression to enhance anti-tumor immune response simul-taneously.Hence,these data posit USP7 inhibitor as an anti-proliferation agent as well as a novel ther-apeutic agent in PD-L1/PD-1 blockade strategy that can promote the immune response of the...
PD-L1: Programmed cell death 1 ligand 1 Her2: Human epidermal growthfactor receptor 2 Wnt/β-catenin pathway: Canonical Wnt/β-catenin pathway Bcl-2 family: B cell lymphoma gene-2 DR5: Death receptor 5 Mcl-1: Myeloid cell leukemia—1 RTKs: Receptor tyrosine kinase PI3K–mTOR:...
Colorectal cancer (CRC) remains a significant global health issue with high incidence and mortality. Yin Yang 1 (YY1) is a powerful transcription factor that acts dual roles in gene activation and repression. High expression level of YY1 has been reporte
Deuterium exchange in USP7 alone was initiated by dilution with 15-fold D2O buffer (pD 7.5), at room temperature. At each deuterium exchange time point (from 10 s to 4 hours) an aliquot from the exchange reaction was removed and labeling was quenched by adjusting the pH to 2.5 with an ...
bintrafusp alfa是一种结合抗PD-L1和TGF-β抑制的双功能融合蛋白,在一项开放标签的1期试验的扩展队列中,30名一线化疗后出现疾病进展的亚洲BTC患者中,bintrafusp alfa显示出临床活性和可控的安全性,客观应答率(ORR)为20%。Hepatology近期刊登...