The ability of surfactant to denature gelatin could be weakened by the addition of urea. The gelatin-induced structural transition has been confirmed by circular dichroism studies. Thiourea affected the micelle-protein interaction in a different manner, which is been explained in light of the ...
When protein quantitation is desired, RIPA buffer is the lysis buffer of choice due to its compatibility with the BCA Protein Assay, although it can denature kinases [5], and can disrupt protein-protein interactions in immunoprecipitation/pull down assays [6]. Urea buffer is another versatile ...
When protein quantitation is desired, RIPA buffer is the lysis buffer of choice due to its compatibility with the BCA Protein Assay, although it can denature kinases [5], and can disrupt protein-protein interactions in immunoprecipitation/pull down assays [6]. Urea buffer is another versatile ...
The ability of surfactant to denature gelatin could be weakened by the addition of urea. The gelatin-induced structural transition has been confirmed by circular dichroism studies. Thiourea affected the micelle-protein interaction in a different manner, which is been explained in light of the ...