The precipitate was then resuspended in 15 ml of PBS. Purification of IgG-containing Immune Complexes by the Use of Protein A Protein A:Sepharose CL-4B (Pharmacia Fine Chemicals, Piscataway, N. J.) ...Siobin, L. I., and M. Sela. 1965. Use of urea in purification of antibodies. ...
Extraction, purification and characterization of the crystallin protein of cataractous eye lens nucleus. The purpose of this study is to separate and identify the crystallin protein present in the nucleus of a human cataractous eye lens. Cataractous lenses wer... Muhammad,Sher,Ayesha,... - 《An...
In subject area: Chemistry Urea is an organic compound, also known as carbamide, with the chemical formula CO(NH2)2. It is a product of protein breakdown in humans and serves as an important indicator of liver and kidney function in clinical diagnostics. Additionally, urea has various industria...
In addition, protein after desalting with the microfluidic device was improved to more than 10% of the relative activity, with a significant improvement of the signal of mass spectrum shown by MALDI-MS. 展开 关键词: micro-fluidic device desalting urea metal ions red fluorescent protein affinity ...
Urea is a powerful protein denaturant via both direct and indirect mechanisms[1]. A potent emollient and keratolytic agent[2]. Used as a diuretic agent. Blood urea nitrogen (BUN) has been utilized to evaluate renal function[3]. Widely used in fertilizers as a source of nitrogen and is ...
2.In the process of purification of arachidonic acid(AA) from fungi lipids by urea inclusion,orthogonal design were used.为提高尿素包合法富集花生四烯酸的效率,通过正交设计对甲醇用量、结晶温度、尿/酯比、降温速度等因素进行分析,发现降温速度对分离效果有显著影响、结晶过程是制约分离效果的瓶颈。 3.Purifi...
was denatured by urea with the concentration ranging from 1.0 to 3.0 mol·L-1.Conclusion High performance hydrophobic interaction chromatography plays an efficient tool in the protein renaturation and the interaction between protein and the HPHIC stationary phase is the driving force for protein ...
Phycocyanin was used as the sole colorant, emulsifier, and antioxidant in emulsion. • Urea was used to improve protein emulsifying capacity and then removed by dialysis. • Algae oil was upcycled in emulsion as a by-product of phycocyanin production. ...
In the present work the application of the method for the urea analysis in milk but initially devised for the urea determination in the blood, is presented. In order to prepare milk, the suitable medium for this analysis the deproteinization and fat removing in the milk sample was made ready...
Urea transporters (UTs) are membrane proteins in the urea transporter protein A (UT-A) and urea transporter protein B (UT-B) families. UT-B is mainly expressed in endothelial cell membrane of the renal medulla and in other tissues, including the brain, heart, pancreas, colon, bladder, bone...