control (QC) sample consisting of equal aliquots from each sample was prepared. Lipidomic analysis was performed using a Thermo Vanquish UPLC system coupled with a Thermo Orbitrap Q Exactive HF-X mass spectrometer operating in the data-dependent acquisition (DDA) mode. Each sample (5 µL) was...
The Q-Exactive HF-X mass spectrometer was used in both positive and negative ion modes with the following conditions: sheath gas at 20 arb; sweep gas at 1 arb; auxiliary gas in positive mode at 5 and negative mode at 7; spray voltage at 3 kV; capillary temperature at 350 ◦C; ...
In the currently study, we have utilized an untargeted lipidomic approach based on UPLC-Q-Exactive Orbitrap-MS to comprehensively investigate the lipid molecular species in the lipid extracts from the spinal cords of eight domesticated animal species. This study represents the first comprehensive lipid...