本研究采用的超高效液相色谱串联四级杆轨道阱质谱(UHPLC-Q-Exactive Orbitrap-MS)技术具有快速、准确、灵敏度高的特点,建立此分析方法对补阳还五汤及其在人血浆和尿液药物化学成分进行快速分析,将为揭示其治疗缺血性脑卒中的物质基础提供更加有利的实验依据。 1仪器与材料 Vanquish UHPLC超高效液相色谱仪、QE-HF-X...
Q Exactive 2.0 SP 2, XCalibur 2.3 and Trace Finder 3.2 softwares (Thermo Fisher Scientific and Dionex Softron GmbH Part of Thermo Fisher Scientific) were used for UHPLC-mass spectrometer control and data processing, respectively. 3.4. LC Parameters A portion of each extract (5 mg) obtained as...
HRMS data were recorded on a Q ExactiveTM hybrid Q–Orbitrap mass spectrometer equipped with a heated ESI source (Thermo Fisher Scientific). The ESI source parameters were set as follows: spray voltage, −2.5 kV/3.0 kV; sheath gas pressure, 40 arb; aux gas pressure, 10 arb; sweep gas ...