High-Throughput Peptide Mapping with the Vanquish UHPLC System and the Q Exactive HF Mass SpectrometerExperimental Conditions - MS Source HESI-II HPLC0.4Martin SamonigKai SchefflerRemco SwartJonathan Josephs
In Beijing, advanced UHPLC-MS/MS techniques were employed using a Vanquish UHPLC system (ThermoFisher, Germany) coupled with an Orbitrap Q ExactiveTM HF mass spectrometer (Thermo Fisher, Germany). Analysis was conducted on a Hypesil Gold column (100 × 2.1 mm, 1.9 μm) with a 17-min ...
UHPLC-Q-Exactive Orbitrap mass spectrometerflavonoidbiotransformationMyricetin is a common natural flavonoid compound with various pharmacological activities. However, the metabolite characterization of this substance is still inadequate. In this study, a simple and rapid system strategy based on UHPLC-Q-...
UHPLC-Q-Exactive Orbitrap MScorrelation analysisGenistin, an isoflavone, has been reported to have multiple activities. However, its improvement of hyperlipidemia is still unclear, and the same is true with regard to its mechanism. In this study, a high-fat diet (HFD) was used to induce a ...
Using Ultra-High-Performance Liquid Chromatography with Q-Exactive Orbitrap Mass Spectrometry technology, we comprehensively investigated the metabolites of daurisoline in Sprague-Dawley rats, following intragastric administration. Data collection and analysis were enhanced through Full Scan MS/dd-MS 2 , in...
In this study, the composition in free and conjugated polyphenols of apricot and peach purees was performed by target and untarget approaches with Q-Exactive quadrupole-Orbitrap mass spectrometer. Apricot purees showed a higher polyphenol content than those of peaches. Between target coumpounds, ...
Sample mass spectrometry signals were collected using a Thermo UHPLC-Q Exactive HF-X Mass Spectrometer equipped with an electrospray ionization (ESI) source operating in positive mode and negative mode, with a mass scanning range of 70–1050 m/z. The optimal process conditions were: source temper...
The method was realized on a high-resolution benchtop Q-Exactive spectrometer using a UHPLC Kinetex® 2.6 µm PS C18 100 Å, LC Column and a gradient elution with water buffered at pH 8 and methanol. The sulfolipids (analyte and internal standard) were quantified in negative ion mode ...
Mass spectrometry analysis was performed on a Q Exactive Quadrupole-Orbitrap mass spectrometer (Thermo Fisher Scientific, Bremen, Germany), which was equipped with a heated electrospray ionization source II (HESI-II) operating in negative ionization mode. The optimised ionization source and MS ...
HRMS data were recorded on a Q ExactiveTM hybrid Q–Orbitrap mass spectrometer equipped with a heated ESI source (Thermo Fisher Scientific). The ESI source parameters were set as follows: spray voltage, −2.5 kV/3.0 kV; sheath gas pressure, 40 arb; aux gas pressure, 10 arb; sweep gas ...