A thin glass cover-slide was mounted on top to monitor the motion of single or aggregated malignant HeLa cells (size 17–30 μm) microscopically through the constricted microchannel at a constant flow rate of 30 μl/h. Quantitative deconvolution of high-speed videographs of a single cell ...
A new method of cancer cell death apart from apoptosis, autophagy and necrosis has been discovered a decade ago. Such a method was termed “ferroptosis” which is an iron-mediated/dependent non-apoptotic but regulated cell death (RCD), specifically for those cells overexpressing small mutated GTPa...
The Power Of Flow Cytometry In Cell Sorting Often, cell sorting is described as trying to find a needle in a haystack. In 2008, David Brown wrote an article oncell sortingfor the Washington Post. In that article, he wrote :“Finding and sorting a few cells from a great mass of them i...
[84] RA Dragovic, JH Southcombe, DS Tannetta, et al. Multicolor flow cytometry and nanoparticle tracking analysis of extracellular vesicles in the plasma of normal pregnant and pre-eclamptic women. Biol Reprod, 2013; 89, 151. [85] FK Marques, FM Campos, OA Filho, et al. Circulating ...
It has been nearly half a century since angioimmunoblastic T-cell lymphoma (AITL) was characterized in the early 1970’s. Our understanding of the disease has dramatically changed due to multiple discoveries and insights. One of the key features of AITL
Flow cytometry techniques using antisera that detect various classes of WBCs are being used to identify cells that are removed versus those that remain using different leukoreduction filters. The result of these white cell characterization studies will be correlated with the results of donor platelet ...
Biotinylation of proteins is a useful tool to label proteins, particularly for flow cytometry experiments and purification techniques. Because biotinylated antigens are commonly used, it is necessary to confirm there is no undesirable interaction between the biotinylated materials and the sensor surface. ...
Differences of these two means of harvesting fundamentally relate to the fact that there is some measured loss of undifferentiated, nucleated cells when examining the total nuclear counts (TNC) and flow cytometry.15 The nucleated cell composition remains essentially the same, with count differences ...
3). A final, integrated assembly of 730.7 Mb was produced in 2,153 scaffolds, comprising 91.3% of the flow cytometry genome size estimate. The final scaffold N50 was 50.4 Mb with more than 90% in 12 pseudomolecules (Supplementary Table 1), presumably corresponding to the 12 SCT ...
As the field of nanomedicine emerges, there is a lag in research surrounding the topic of nanoparticle (NP) toxicity, particularly concerned with mechanisms of action. The continuous emergence of bacterial resistance has challenged the research community