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Primary human T cells were cultured in complete RPMI 1640 supplemented with 100 U/ml recombinant human IL-2 (PeproTech, 200-02). All mammalian cells were cultured at 37 °C in a humidified 5% CO2 incubator. Gene delivery methods General plasmid transfection in HEK 293 T cells was ...
Using immunohistochemistry, integrin-αVβ5 indeed expressed in OGD-injured hippocampal astrocytes, and seemed to express more in LIPUS-treated OGD astrocytes (Fig. 7C). Moreover, we found LIPUS enhances the mRNA expression of integrin-αV and integrin-β5 in OGD-injured hippocampal astrocytes (...
Indeed, it would be useful to know the specific parameters required to trigger channel opening in PGVs-mediated ultrasound stimulation. However, we were able to collect evidence of the role of mechanosensitive ion channels through calcium imaging by using various blockers, and collected real-time ...
Primary human T cells were cultured in complete RPMI 1640 supplemented with 100 U/ml recombinant human IL-2 (PeproTech, 200-02). All mammalian cells were cultured at 37 °C in a humidified 5% CO2 incubator. Gene delivery methods General plasmid transfection in HEK 293 T cells was ...
The thyroglossal duct cyst (TGDC) is the most common congenital neck mass, accounting for 70–75% of all congenital neck masses. Although the Sistrunk operation has been used as a standard of treatment, it is accompanied by a considerable surgical burden
It has been established that application of ultrasound can indeed give significant process intensification benefits in terms of reduced treatment times, better quality products as well as reduced requirement of chemicals. The optimum considerations for design of sonochemical and hydrodynamic cavitation ...
(AUROC: 0.974–0.990). We also explored the impact of training dataset size on the performance of AI system. We observed that more training data led to a better AUROC (Supplementary Table1). In addition, we evaluated the AI system on the external test set (BUSI dataset)40. Even though ...
UCLA—Caltech Medical Scientist Training Program, David Geffen School of Medicine, Los Angeles, CA 90095, USA 2 Department of Neurosurgery, University of California, Los Angeles, CA 90095, USA 3 Department of Psychiatry and Biobehavioral Sciences, University of California, Los Angeles, CA 90095, ...
In individuals with NSLBP, TrA contraction has been reported to be significantly delayed in activation during a physical task or movement, indicating a potential for reduced spinal stability as well as underlying motor control problems in the lumbopelvic region [6]. Indeed, it has also been repor...