The BMP-2/4 monoclonal (Santa Cruz Biotechnology, sc-137087) and negative IgG antibodies were used as capture antibodies in the Co-IP reactions, and Western blot analysis of the eluted proteins were performed u
Rabbit mAb to Actin (Abclonal AC026, 1:50,000 for IB), rab- bit polyclonal anti-Myc (A-14) (Santa Cruz Biotechnol- ogy, sc-789; 1:2000 for IB), mouse monoclonal anti-Myc (9E10) (Santa Cruz Biotechnology, sc-40; 1:2000 for IB), protein A-HRP (Sigma-Aldrich, GENA9120; 1:...
The following antibodies were used: anti- BMP-2 (Santa Cruz, sc-6895), anti-Runx2 (M70, Santa Cruz, sc-10758) anti-total Ucma/GRP antibody (Cterm-GRP, GenoGla Diagnostics, Faro, Portugal), HRP conjugated secondary antibodies and DAB or Nova-RED substrate (Vector Labs, Amsterdam, the ...
Muskelin Santa Cruz Biotechnology Cat# SC-398956;RRID:AB_2737249 Muskelin Proteintech Group Cat# 14735-1-AP;RRID:AB_2142504 MAEA Proteintech Group Cat# 28363-1-AP;RRID:AB_2881122 ARMC8 Proteintech Group Cat# 12653-1-AP;RRID:AB_2059154 WDR26 Bethyl lab Cat# A302-245A;RRID: AB_1730876 p70...
Toxicology Working Group: Antonia Socias (Hospital Son Llàtzer, Palma), and Jaume Baldirà (Hospital de la Santa Creu i Sant Pau, Barcelona). Transfusions and Hemoderivatives Working Group: María Gero Escapa (Hospital Universitario de Burgos, Burgos); Manuel Quintana Díaz (Hospital Universitario...
Dilution CX3CR1 Novus, 76,949; Abcam, 217,291 1:1000 UCHL1 Novus, nb600-1160; Protein- 1:1000 tech, 14730-1-AP FLT1 Novus, nb600-1004 1:1000 ALIX CST, 2171 S 1:1000 TSG101 Proteintech, 28283-1-AP 1:1000 CD63 Proteintech, 67605-1-Ig 1:1000 CD9 Santa Cruz, SC-13,118...
ERK1/2 siRNA (sc-29308, sc-35336, Santa Cruz, USA), IP3R1 siRNA (sc- 42476, Santa Cruz, USA) or negative control siRNA (sc-37007, Santa Cruz, USA) were transfected to hepatocytes using OPTI-MEM (Invitrogen, USA) and Lipofectamine RNAi MAX reagent (Invitrogen, USA) respectively. ...
(Santa Cruz Biotechnologies 47778, Dallas, TX, USA), UCHL1 (Protein Tech 14730-1-AP, Rosemont, IL, USA), Myogenin (Santa Cruz 52903), MyoD (Santa Cruz 377460), GAPDH (Santa Cruz 166574), DRP1 (Cell Signaling technology 8570S, Danvers, MA, USA), P-Pink1 (Cell Signaling 89010S), ...
1. Introduction Sucrose is one of the main products of photosynthesis and the most common trans- ported sugar in plants, and it is also an easily assimilated macronutrient that provides a car- bon or energy source for insects to fulfil the requirement of physiological metabolism [1,2]. ...