TRE17 encodes a ubiquitin-specific protease (USP) and a TBC (TRE2-Bub2-Cdc16) domain that promotes activation of the Arf6 GTPase. Here we report that TRE17 potently inhibits the maturation of MC3T3 pre-osteoblasts in a USP-dependent and Arf6-independent manner. Notably, we find that TRE...
Ubiquitin-specific protease-6 (UBP6) in Saccharomyces cerevisiae was expressed in Escherichia coli and purified from the cells using 125 I-labeled ubiquitin-αNH-MHISPPEPESEEEEEHYC as a model substrate. The purified UBP6 behaved as a 58-kDa under both nondenaturing and denaturing conditions, ind...
A ubiquitin-specific protease which cleaves ubiquitin from any protein or peptide to which ubiquitin is joined and the gene encoding the protease are disclosed. The protease specifically cleaves the peptide bond in a fusion of ubiquitin to a protein or peptide between the carboxyl-terminal amino aci...
As the largest family of DUBs, USPs bear both similarities with, and critical differences from, the other cysteine protease DUBs. All DUB catalytic domains contain a primary ubiquitin-binding site that enables numerous interactions with ubiquitin in the distal region of a polyubiquitin chain [6]. ...
Protein ubiquitination and deubiquitination are reversible processes, which can modulate the stability, activity as well as subcellular localization of the substrate proteins. UBIQUITIN-SPECIFIC PROTEASE (UBP) protein family participates in protein deubiquitination. Members of UBP family are involved in a va...
Zhong B, Liu X, Wang X, Li H, Darnay BG, Lin X, Sun SC, Dong C: Ubiquitin-specific protease 25 regulates TLR4-dependent innate immune responses through deubiquitination of the adaptor protein TRAF3. Sci Signal. 2013, 6: ra35-10.1016/j.sigpro.2012.06.015. Article PubMed Google Scholar...
Activity-based ubiquitin-specific protease (USP) profiling of virus-infected and malignant human cells. The family of ubiquitin (Ub)-specific proteases (USP) removes Ub from Ub conjugates and regulates a variety of cellular processes. The human genome contain... Huib,Ovaa,Benedikt,... - 《...
USP10 also antagonizes c-myc transcription through deubiquitination of SIRT6, a histone deacetylase, and NF-κB signaling through deubiquitination of NEMO [52]. The function of USP25 in normal physiology and cancer is unknown, although recent studies suggest this protease plays a regulatory role in...
Ubiquitin-specific protease 14 AS: Aortic stenosis AVR: Aortic valve replacement TAVI: Transcatheter aortic valve implantation FC: Fold change GO: Gene Ontology BP: Biological processes CC: Cellular components MF: Molecular functions KEGG: Kyoto Encyclopedia of Genes and Genomes GSEA: ...
cysteine-dependent proteases, an analogous mechanism of action of the cysteine protease papain, which features three highly conserved subdomains resembling the fingers, thumb, and palm of the right hand [19,20]. USPs are characterized by the presence of a conserved catalytic domain known as the ...