U251 cell morphology was observed by a light microscope. The time ofdoubling cells was detected by the cell count technique. The U251/TMZ multidrug resistance was assayed by MTT. The changes in U251cells and U251/TMZ cells cycles were detected by flow cytometry. RT-PCR assay resistant cell...
U251人神经胶质瘤细胞 U251人神经胶质瘤细胞 Cat Number：KG050 For Research Use Only 一、组成:二、客户自备试剂:1、PBS (凯基货号：KGB5001)2、Complete growth medium (凯基货号：KGM 12800-500)3、0.25% （W/V） Trypsin-0.53mM EDTA (凯基货号：KGY0012)三、细胞简介:
colonies.Amongthesecolonies.thetightcoloniesshowedthepoorestdifierentiation.U251cellsoftight coloniespossessedthepropertiesofseIf-renewalandmultiplelineagedifferentiation.Moreover.theywere foundtobeabletoexpressCDl33andnestin.Conclusions U251cellcoloniesvariedinmorphologyand difierentiation.andthetightcoloniesmightbe...
表2 槲皮素作用48 h后对U251 胶质瘤细胞周期分布的影响 Table 2 Effect of quercetin on cell cycle of U251 cel ls after 48 h of treatment Quercetin (μmol/ L) n G0/ G1 (%) (S+G2)/ M(%) 0 3 90.60±1.14 8.52±2.23 40 3 80.17±2.74 * 19.54±3.89 * 80 3 78.58±1.45 * 21.03...
胶质瘤凋亡Objective To investigate the molecular basis for apoptosis of glioma by tubeimoside-1 ( TBMS Ⅰ) in U251 cell line.Methods U251 cells were exposed to different concentrations of tubeimoside-1, cell proliferative activity was evaluated using MTT assay.The changes of nuclei morpho...
cell apoptosis,Transwell was performed to assay invasion changes,and scanning electron microscopy was applied to observe cell morphology changes after transfection.RESULTS:RT-PCR and Western Blot screen of a MOESIN-139 fragment of the best silence.siRNA transfected U251 cell growth was slowed down,in...
An in-verted microscope was used to observe the changes of cell number,morphology and activity. AnnexinV/ PI was used to measure the in-duction of cell apoptosis caused by Bufalin. Results:Bufalin at different concentrations(0. 001 - 10. 0μmol·L - 1 )inhibited the pro...
cell proliferation and calculate inhibition ratio by means of CCK-8 method.The morphology of the nucleus was observed by Hoechst 33342/propidium iodide double staining under fluorescence microscope.Flow cytometry results showed the cell cycle.Reverse transcription-polymerase chain reaction was used to ...
Retinoic acid and the cyclin dependent kinase inhibitors synergistically alter proliferation and morphology of U343 astrocytoma cells. We have characterized the expression and activity of the cell cycle regulatory machinery and the organization of the cytoskeleton of the p16Ink4a-deficient... Dirks,B ...
cellsofthemcallbeinducedtodifferentiateandadherenttothe bottomoftheculture plates.Thegrowingpatterns ofU251 glioma cell lineCanbeconverted bychanging theculture mediums.Cryopreservation couldbealleffectivemeansto preserveBTSCs,butvigour ofthecells willbedecrease obviously.There aresomedifferencein morphology, pro...