Each gene was quantified in triplicate with a standard curve and negative control. 2.4. High-throughput sequencing and bioinformation analysis iDNA and eDNA extracted were used for 16S rRNA gene high-throughput sequencing. The V4 region of the bacterial 16S ribosomal RNA gene was amplified by PCR ...
The bacterial expression plasmid pET3a containing the coding sequence of ACO (Diox1, GenBankTM BAA18428.1) from Synechocystis sp. PCC 6803 was transformed into the T7 express BL21 E. coli strain (New England Biolabs, Ipswich, MA). Cells were grown at 37 °C to an A600 nm of ∼0.6 at...
Changes in bacterial community had the greatest impact on ARG succession. Nevertheless, microorganism community was also indirectly affected by the soil physical and chemical properties (pH, TOC and MC) and plant growth. No changes in microbial community were observed following the addition of wuyi...
(and conductivity) had negative correlations with alpha-diversity indices of the bacterial communities, and phosphate concentration and turbidity had positive correlations, while the other factors had almost no correlations, which partially explains the region-specific diversity. These results also suggest...
Responses of soil and earthworm gut bacterial communities to heavy metal contamination. Environ. Pollut. 2020, 265, 114921. [Google Scholar] [CrossRef] [PubMed] Xu, C.; Pu, J.; Wen, B.; Xia, M. Potential Ecological Risks of Heavy Metals in Agricultural Soil Alongside Highways and Their...
Salinity and nutrient modulate soil bacterial communities in the coastal wetland of the Yellow River Delta, China. Environ. Sci. Pollut. Res. 2021, 28, 14621–14631. [CrossRef] 2. Pan, C.; Liu, C.; Zhao, H.; Wang, Y. Changes of soil physico-chemical properties and enzyme activities...
Contrasting Soil pH Effects on Fungal and Bacterial Growth Suggest Functional Redundancy in Carbon Mineralization▿ †. Appl. Environ. Microbiol. 2009, 75, 1589–1596. [Google Scholar] [CrossRef] [PubMed] [Green Version] Paterson, E.; Thornton, B.; Midwood, A.J.; Osborne, S.M.; Sim...
second collection. The culture process and results are shown inFigure 2b,c. The plate counting method was used to count the fungal colonies after culture. Then, according to the formula (5), the number of bacterial colonies was converted into the concentration of culturable fungi in the ...