Several benign and malignant skin tumors were analyzed for the presence of human papillomavirus (HPV) DNA. By hybridization with different HPV DNA probes under non-stringent conditions (Tm -40° C), two tumors were found to contain HPV-specific DNA sequences in high copy numbers: (1) a kera...
Genes specific for Escherichia coli O157:H7 and E. coli O26 serotypes as molecular probes for rapid identification.In this study, the O-antigen was cloned from E. coli O26 and E. coli O157 into the cosmid vector, pCosEMBL2. Screening of the cosmid library was done by Western analysis ...
- 《Molecular & Cellular Probes》 被引量: 116发表: 1993年 Distribution of both rotavirus VP4 genotypes and VP7 serotypes among hospitalized and nonhospitalized Israeli children. Human rotaviruses belonging to genotype P9, of probable feline origin, which included both G3 and G1 serotypes, were...
Many of the molecular changes evoked during adult neurogenesis can now be ascribed to specific cellular events in the OSN cell lineage, thereby defining new stages in the development of these neurons. Most notably, the patterns of gene expression in immature OSNs changed in a characteristic fashion...
Mechanisms of anesthetic action (numbing) are well studied and much is known regarding the molecular events subserving numbing anesthesia. As it relates to the alkylamides and eugenol, there are several mechanisms mediating the anesthetic action of these compounds. When ingested, alkylamides and ...
Tumor invasiveness reflects many biological changes associated with tumorigenesis, progression, metastasis, and drug resistance. Therefore, we performed a systematic assessment of invasiveness-related molecular features across multiple human cancers. Mul
Single-cell RNA sequencing data can unveil the molecular diversity of cell types. Cell type atlases of the mouse spinal cord have been published in recent years but have not been integrated together. Here, we generate an atlas of spinal cell types based on single-cell transcriptomic data, unify...
The primary antibodies used were mAb22C10 (1:50, DSHB donated by Seymour Benzer), Rabbit-anti-Repo (1:1000, Susinder Sundaram), Rat-anti-Repo (1:500, Susinder Sundaram), Rabbit anti-GFP (1:10,000, Molecular Probes). Secondary antibodies used were: Alexa 488 goat anti-rabbit, Alexa ...
20 May 2016 Glycosyltransferase Gene Expression Profiles Classify Cancer Types and Propose Prognostic Subtypes Jahanshah Ashkani1,2 & Kevin J. Naidoo1,2 Aberrant glycosylation in tumours stem from altered glycosyltransferase (GT) gene expression but can the expression profiles of these signature ...
Recently, chemical screening from natural sources and compound libraries has led to the rapid discovery of enzyme inhibitors. To create more useful inhibitors with high enzyme selectivity, and molecular probes for analyzing the precise mode of actions for enzymes, synthetic approaches based on natural ...