Radio-ligand binding assays and Western blot experiments were performed in order to investigate their presence in sheep cardiac and rat liver nuclear envelopes. Ryanodine receptors (RyR) were not detected in liver nuclear envelopes by either binding assay or Western blot analysis. However, cardiac ...
Assuming that you have two purified binding partners ready to go, it is now time tochoose one your of interactantsto be immobilized to a sensor chip of appropriatesurface chemistry– this will be your ligand. Your other interactant (the analyte) is then injected and flows over your immobilize...
Development of high throughput screening assays using fluorescence polarization: nuclear receptor‐ligand‐binding and kinase‐phosphatase assays. J Biomol... Fluorescence polarization (FP) has been used to develop high throughput screening (HTS) assays for nuclear receptor-ligand displacement and kinase in...
It encodes the neuropeptide kisspeptin or metastin, the natural ligand of the kisspeptin receptor. The mutation is inherited as variable expressivity or polygenism from unaffected maternal factors, such as the mother and grandmother [51]. The signs of pubertal development in male patients appear ...
Here, we present a ligand-based proximity labeling approach to interrogate the native cell membrane interactome for the GLP-1 receptor after agonist simulation. Our study identified several unreported putative cell membrane interactors for the endogenous receptor in either a pancreatic β cell line or ...
Therefore, we speculated that several genes from the 24-gene signature might generate unexpected effect on the invasiveness level of these tumor types, thus leading to the opposite prognostic results. For example, INHBA (inhibin βA), a subunit of a ligand of the transforming growth factor-β ...
For each cell type, we cataloged alterations in gene expression, molecular pathways, transcriptional networks, ligand–receptor interactions and senescence status. Our analyses identified gene signatures, demonstrating that heterochronic parabiosis regulates several hallmarks of aging in a cell-type-specific ...
HPV type of groups A1, A2, C1, C2, or D and another portion of the capture oligomer serves as one of a binding pair (ligand) to immobilize the captured HPV RNA on a solid support to facilitate separation of the captured HPV RNA from other sample components before the amplification step....
Using in vitro reconstitution, we demonstrate that single-isotype α1β4 GDP-tubulin lattices are stabilised and expanded by 10 µM taxol, as reported by accelerated microtubule gliding in kinesin motility assays, whereas single-isotype α1β3 GDP-tubulin lattices are stabilised but not ...
TAZ induces growth factor-independent proliferation through activation of EGFR ligand amphiregulin. Cell Cycle 2012;11: 2922–2930. 29 Liu C, Huang W, Lei Q. Regulation and function of the TAZ transcription co-activator. Int J Biochem Mol Biol 2011;2:247–256. 30 de Cristofaro T, Di Palma...