Sigel, E., Stephenson, F. A., Mamalaki, C. and Barnard, E. A. The purified GABA/benzodiazepine/barbiturate receptor complex: four types of ligand binding sites, and the interactions between them, are preserved in a single isolated protein complex. J. Receptor Res. 4 (1984) 175–188...
Biochemical coregulator interactionLanthaScreen® NR Co-regulator Interaction AssayTR-FRET based assay to evaluate recruitment or displacement of a fluorescein-labeled coregulator peptide. Evaluated conformational changes of nuclear receptors upon liga...
As readouts, we applied a combination of single-cell mass cytometry (CyTOF)65, imaging mass cytometry (Hyperion Imaging System)66 and energetic metabolism by profiling translation inhibition (SCENITH)67, as well as functional assays to monitor immune cells, and subjected serum to large-scale ...
Using in vitro reconstitution, we demonstrate that single-isotype α1β4 GDP-tubulin lattices are stabilised and expanded by 10 µM taxol, as reported by accelerated microtubule gliding in kinesin motility assays, whereas single-isotype α1β3 GDP-tubulin lattices are stabilised but not ...
Here, we present a ligand-based proximity labeling approach to interrogate the native cell membrane interactome for the GLP-1 receptor after agonist simulation. Our study identified several unreported putative cell membrane interactors for the endogenous receptor in either a pancreatic β cell line or ...
(ligand). The ligand can be an antibody, protein or antigen, DNA/RNA probe, or any other molecule with an affinity for the desired target (Figure 1). Ready-to-use Dynabeads products and kits are available for many applications. For assays that require flexibility, a range of Dynabeads ...
differences in neurochemical ligand and receptor combinations [230]. The concept of regional heterogeneity in cerebrovasculature molecular profiles is reinforced by the non-uniform yet stereotypic vascularization of the brain [35]. White matter regions are considerably less vascularized compared to gray ...
Here, we present a ligand-based proximity labeling approach to interrogate the native cell membrane interactome for the GLP-1 receptor after agonist simulation. Our study identified several unreported putative cell membrane interactors for the endogenous receptor in either a pancreatic β cell line or ...
Prorocentrolide-A, in contrast to ACh (the endogenous agonist of nAChRs), had no agonist properties on both α12β1γδ and α7 nAChRs incorporated or expressed in the oocyte membrane, respectively, but blocked the inward current evoked by ACh. Ligand-binding assays, performed on membranes ...
Three types of white perch (Morone americana) vitellogenin (VtgAa, VtgAb, and VtgC) were purified, labeled with digoxigenin (DIG), and subjected to Vtg receptor (Vtgr) binding assays in 96-well plates coated with perch ovarian membrane proteins or to ligand blotting procedures. Binding specifi...