The disclosure provides a method of measuring the relative RBP-binding affinity of a liposome in a sample. In some aspects, the method comprises contacting the sample with a modified RBP associated with an immobilized surface, wherein an exterior surface of the liposome comprises a retinoid or a...
Ligand binding assays routinely employ fluorescently-labeled protein ligands to quantify the extent of binding. These ligands are commonly generated through chemical modification of accessible lysine residues, which often results in heterogeneous populat
Provided are rapid and sensitive cell-free assay methods for detecting and/or measuring specific bimolecular or higher order interactions via reassembly of a split monomeric reporter protein, and methods of detecting or identifying modulators of such interactions by the effect on the signal provided by...
Protein-ligand binding investigated by a single nanoparticle TERS approach. We report TERS imaging of individual 50 nm, biotin-labeled gold nanoparticles bound to a streptavidin-derivatized glass slide. Individual gold nanoparticle... SL Carrier,CM Kownacki,ZD Schultz - 《Chemical Communications》 被引...
c, Heatmap visualizes the effect of mutations in the ligand binding pocket of mTAAR1 in response to T1AM, as determined by the CAMYEL assay. The heatmap is coloured according to the value of ΔpEC50 (ΔpEC50 = pEC50 of mutant - pEC50 of WT) and Emax (relative WT%). ND, ...
DEL for Covalent Ligand Discovery DNA-Encoded Library (DEL) for Compound Optimization & Expansion DEL for Enzymes with Specific Mode of Action (MoA) DEL for Protein-Protein Interaction DEL for RNA DEL for DNA- and RNA-Binding Proteins DEL for GPCRs DEL for Protein Degradation DNA-...
Computational Design of Ligand Binding Proteins Chapter © 2017 The Framework of Computational Protein Design Chapter © 2017 Achievements and Challenges in Computational Protein Design Chapter © 2017 Main Protein interactions have crucial roles in biology, and general approaches to design protein...
The ratio of the molecules of EmrE to the molecules of ligand introduced per injection had also been tested in the range of 0.1:10. It was found that a ratio in the range of 10:4 was suitable for measuring the heats evolved from EmrE binding to any of the substrates in any of the ...
expression systems, various novel target proteins and multi-protein complex assembly, as well as refolding and purification skills. We focus on novel protein targets and customized Gene-to-Protein projects, whether for crystallization purposes, screening, assay development or general drug discovery ...
ligand-binding sites of 4Fe–4S obtained from BioLiP, as shown by the ROC curve.dgrad-CAM profiles for predicted DNA binding and metal ion binding functions mapped onto the structure of an unannotated zinc finger protein (PDB Id: 1MEY) found in Escherichia coli; the corresponding ROC curves...