This chapter discusses several types of liquid column chromatography. In adsorption chromatography, the retention of the solute is a consequence of the interaction with the surface of the solid adsorbent. The adsorbent surface has a rigid structure making this type of chromatography uniquely useful for...
R. E. PaulsTaylor & Francis GroupJournal of Liquid ChromatographyMcCoy KW, Pauls RE. Comparison of commer- cial column types in liquid chromatography. J Liq Chromatogr 1982; 5: 1869-97.McCoy, R.W. and Pauls, R.E. (1982) J. Liquid Chromatogr , 5 , 1869....
Overview of Chromatography:In chromatography, a mixture of substances is dissolved in a liquid or gas medium, known as the mobile phase. The solution containing the mixture of substances is percolated through a column containing a solid but porous matrix, c...
The mobile phase is one of two phases associated with chromatography. During the mobile phase in chromatography, the analyte, carried by the eluent, is actively added to the chromatography column. The second phase is known as the stationary, or immobilized, phase. The stationary phase in chromato...
Detailed notes on HPLC aka High Performance Liquid Chromatography, that cover principle, types, parts, uses, and applications.
The oxalate content was measured by the high performance liquid chromatography (HPLC) method with an anion exchange column using 0.85 mM sodium bicarbonate and 0.9 mM sodium carbonate as a mobile phase. The results showed that the highest of oxalate content was found in black tea (1.36–4.42 ...
The capsid serotype helper plasmids also contain all necessary helper function in addition to rep and cap in trans for their respective capsid serotype.40,41 Recombinant virus was then purified by iodixanol step gradients and Sepharose Q column chromatography as previously described41 and suspended in...
et al. " Role of surface groups in affecting the chromatographic performance of certain types of fused-silica glass capillary column " Journal of Chromatography, 1984, pp. 129-142, vol. 289.Role of surface groups in affecting the chromatographic performance of certain types of fused-silica glass...
The recombinant virus DNA bacmid was isolated from overnight culture using MidiPrep (Qiagen) reagents (without the use of a column). The DNA was precipitated overnight in absolute isopropanol at −20 °C, pelleted the next day at 14,000 × g for 15 min, washed in 70% ...
Purification of recombinant adeno-associated virus vectors by column chromatography and its performance in vivo Hum Gene Ther, 11 (2000), pp. 2079-2091 CrossrefView in ScopusGoogle Scholar 40 JH Wolfe, MS Sands Murine mucopolysaccharidosis type VII: a model system for somatic gene therapy of ...