Various types of column packings were evaluated for use in the gas chromatography determination of ppm concentrations of ethylene glycol in aqueous systems. The columns were evaluated by determining specific retention volumes and observing peak shapes for progressively smaller concentrations of ethylene ...
Overview of Chromatography:In chromatography, a mixture of substances is dissolved in a liquid or gas medium, known as the mobile phase. The solution containing the mixture of substances is percolated through a column containing a solid but porous matrix, c...
Size Exclusion Chromatography (SEC) - separates analytes when going down the column and some get stuck in stationary phase pores. Small particles are the ones trapped while large ones elute first. Affinity Chromatography - purifies and concentrates specific species. The sample is applied to the colu...
Helium was employed as a carrier gas, with a flow speed of 0.8 mL/min. Two parallel chromatographic columns including MXT-5 and MXT-1701 (20 m × 0.18 mm I.D. × 0.4 μm; Restek, Beijing, China) were used to separate the volatile compounds. The initial column temperature was held ...
Plant roots release a variety of organic compounds into the soil which alter the physical, chemical and biological properties of the rhizosphere. Root exudates are technically challenging to measure in soil because roots are difficult to access and exuda
Dietary fibre-induced satiety offers a physiological approach to body weight regulation, yet there is lack of scientific evidence. This experiment quantified food intake, body weight and body composition responses to three different soluble fermentable dietary fibres in an animal model and explored underl...
Column:HP-SMS (Agilent 19091S433:30mx0.25 μm x 0.25 μm). The specific operation and parameter settings followed the method of Burton et al. (Burton et al., 1989) 2.2.2. Determination of conventional chemical compositions The indices of main chemical compositions were total ...
Describe how the separation of mixture is done using column chromatography. What types of species can be separated by HPLC, but not by GC? 1)What's the Tyndall Effect and whats one example of this effect? 2) Given an unknown mixture consisting of two or more substances, how could we det...
GC separation was conducted on a capillary column TR-5MS (30 m × 0.25 mm × 0.25 μm, Thermo Fisher, USA). The injector temperature was controlled at 230 °C and the split rate of the injector was 1:50. Helium was used as a carrier gas at a ...
Compared with the liquid-liquid extraction and solid phase extraction, this extraction method can avoid loss of volatile components in removing solvent and at the same time reducing the extraction noise and has higher sensitivity and faster analysis. This method can protect the analytical column from...