Foreign nations began using chemiluminescent chemicals to mark antigens or antibodies around the end of the 1980s, resulting in the development of luminescence immunoassay technology. Luminescence immunoassay (LIA) is a term that refers to chemiluminescence immunoassay (Chemiluminescence immunoassay, CLIA)....
Alopecia presents with various symptoms depending on its type. The most common sign is the thinning of hair, which in men often begins on the forehead in a pattern resembling the letter M. Women generally experience a broadening of the part in their hair. Some cause patchy hair loss, sudde...
In the indirect approach, detection of the immune complex is achieved through the labeling of either the antibody or the antigen depending on the immunoassay format (sandwich type, competition, and capture) with signaling molecules. Most often, optical detection methods (fluorescence chemiluminescence, ...
belongs to a group of disorders where there is some form of inflammation “-itis” of the thyroid gland. It is basically an autoimmune destruction of the thyroid gland, which typically progresses gradually to hypothyroidism, or state of too low “hypo-“ thyroid hormones. In fac...
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The immunoreactive proteins on the membrane were then visualized using an enhanced chemiluminescence substrate (RPN2106, Amersham). 2.4. Enzyme linked immunosorbent assay (ELISA) For virion ELISA, assay plates (9018, Costar, Washington, D.C., USA) were coated with the antibody 4G2, which could ...
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(Amersham, NA934). Primary antibody was incubated for 30 minutes at room temperature, as was the secondary antibody. The immunocomplexes were detected by chemiluminescence using Supersignal® chemiluminescent substrate reagents (Pierce) according to the manufacturer's instructions. Arrow indicates sGP ...
such as an antibody functioning as a detection antibody. The detectable label can for example be a direct label, which may be an enzyme, oligonucleotide, nanoparticle chemiluminophore, fluorophore, fluorescence quencher, chemiluminescence quencher, or biotin. Test kits may optionally include any additio...