While it is straightforward to define the reference range for an NGS test as the range of normal sequence variants within a population [1], this definition is problematic because normal variation differs among various populations, may be biased by inaccurate disease associations and genotype–...
Hebei University, Baoding 071002, China; 2 Department of Chemistry, Tsinghua University, Beijing 100084, China; 3 Beijing Chemclin Biotech Co., Ltd., Beijing 100094, China.Microplate chemiluminescence enzyme immunoassay for the quantitative evaluation of carbohydrate antigen 72-4 in human serum[J]. ...
We developed a two-site chemiluminescence immunoassay for human vascular endothelial growth factor (VEGF). The assay recognized both VEGF121 and VEGF165 isoforms, but had no detectable cross-reactivity with platelet-derived growth factor or placenta growth factor. The range of detection was between 30...
(24). The performance of these procedures compares very favourably withexisting methods of immunoassay based on 125i as the label and indicates the generalapplicability of the technology.CONCLUSIONSChemiluminescence immunoassay offers a viable and logical alternative to a range ofanalytical procedures based...
priming agent, an opsonified oxidative metabolism stimulating agent and a chemiluminigenic substrate, measuring the chemiluminescence response of the sample, determining the chemiluminescence response per phagocyte of the sample and comparing the response per phagocyte with that of the normal range of ...
In the current analysis of paired plasma and CSF obtained at time of diagnostic LP, we demonstrated excellent performance of a novel high-sensitivity plasma p-tau217 ECL immunoassay for the detection of Aβ positivity in individuals presenting to specialist memory services with MCI/mild dementia– ...
The proposed method is highly sensitive and selective to adenine and adenosine. Adenine can be determined in the range 1.5 × 10 4 5.0 × 10 7 M. The detection limit for adenine is 1.9 × 10 7 M (19 pmol per assay).关键词: CHEMILUMINESCENCE ADENINE ADENOSINE NUCLEIC ACID BASES ...
The ECL intensity of the immunosensor decreases linearly with the logarithm of CA15-3 concentration in the range of 0.01–100 U/mL with a detection limit of 0.004 U/mL. This new method should improve the ECL signal of the immunosensor for detection of CA15-3....
The workflow of the immunoassay was found to be advantageous in certain instances when compared to these quantification methods. Since the reagents used for CLIC are common to other immunoassays with no need for specialized equipment, and due to the good linearity, dynamic range and signal ...
(I). ECL analysers forimmunoassayandnucleic acid probeassays have been commercially available since the mid-1990s, beginning with the ORIGEN Analyser [126,127] from IGEN International (now owned by Roche Diagnostics). This instrument and others based on ORIGEN technology are designed to detect ...