For migration assay, we used the transwell chambers with inserts that contained the same type of membrane but without the matrigel coating. Wound-healing assay Cells were plated in 60 mm culture dish and grown to 80% confluence in complete medium. A ‘wound’ was made by scraping with a ...
Dong CJ, Beis K, Nesper J, Brunkan-LaMontagne AL, Clarke BR, Whitfield C, Naismith JH (2006) Wza the translocon forE. colicapsular polysaccharides defines a new class of membrane protein. Nature 444:226–229 (PDB id 2J58) CASPubMedGoogle Scholar Doyle DA, Morais Cabral J, Pfuetzner ...
An antibody capable of binding to a sugar chain-modified HEG1 protein obtained from mesothelioma.辻 祥太郎影山 泰平鷲見 公太辻 真紀子松浦 利絵子吉原 光代今井 浩三
The protein product of the pseudorabies virus (PRV) Us9 gene is a phosphorylated, type II membrane protein that is inserted into virion envelopes and accumulates in the trans-Golgi network. It is among a linked group of three envelope protein genes in the unique short region of the PRV genom...
背景说明:NKG2-A/NKG2-B Type II Integral Membrane Protein contains 1 C-type lectin domain and belongs to the killer cell lectin-like receptor family. The killer cell lectin-like receptor family is a group of transmembrane proteins preferentially expressed in NK cells. Members of this proteins is...
The membrane-binding matrix (MA) domain of the human immunodeficiency virus type 1 (HIV-1) structural precursor Gag (PrGag) protein oligomerizes in solutio... A Alfadhli,D Huseby,E Kapit,... - 《Journal of Virology》 被引量: 83发表: 2007年 Peptide-bound Major Histocompatibility Complex Cl...
The regulation of biological activity at the cell surface is achieved through a variety of processes including recycling, shedding, and protein-protein and protein-membrane interactions. While protein activities are often studied in isolation, methods by which activities could be evaluated in membrane-...
Pharmacoproteomics of a metalloproteinase hydroxamate inhibitor in breast cancer cells: dynamics of membrane type 1 matrix metalloproteinase-mediated membrane protein shedding Mol Cell Biol, 28 (2008), pp. 4896-4914 View in ScopusGoogle Scholar [192] T. Takino, N. Koshikawa, H. Miyamori, M. Tan...
Through both mechanisms, the ERGIC–ERES contact promotes formation of the ERGIC-derived COPII vesicle, a membrane precursor of the autophagosome. The ERGIC–ERES contact is physically and functionally different from the TFG-mediated ERGIC–ERES adjunction involved in secretory protein transport, and ...
Figure 2: Structural elements of CamAlstR-C. (a) The sequence motifs that were detected in combination of PDBsum and MotifScan. The total protein sequence contains 15 helices (purple ribbons) constituting 7 transmembrane domains and one intracellular C-terminal helix. The receptor has a typical ...