The results revealed that the separation of dipeptides was possible using the beta-cyclodextrin column. Changing the percent methanol in the mobile phase from 15 to 10 percent improved the separation only slightly. Changing the pH of the mobile phase affected the peak shape and retention times ...
1. 色谱柱 色谱柱是HPLC的关键部件,它使用各种填料将混合物中的不同分子分离出来,并出现在分离的不同位置。常见的色谱柱有反相、离子交换、正相、大小排除等。 2. 色谱柱恒温器 色谱柱恒温器(column heater)可以保持色谱柱的恒温,以提高分离效果和可重现性。 四、泵 泵是HPLC的...
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The column contains analyte-specific or group-specific affinity ligand. The analyte recognizes and binds to the ligand in a very specific manner. Another type of HPLC is called denaturing HPLC (DHPLC), which is used in determining single nucleotide polymorphisms (SNPs), the most common type of ...
A solution of Togni reagent (224.5 mg, 0.68 mmol, 1.7 equiv) in anhydrous i-PrOH (1 + 1 mL) was added. The resulting reaction mixture was stirred at ambient temperature for 17 h and concentrated by rotatory evaporation. The crude mixture was purified by flash column chromatography (EtOAc/...
活化色谱柱是一种常见的分离技术,它可以通过固定相和移动相之间的反应来分离化合物。在活化色谱柱中,样品通常通过色谱柱由流动相带动;因此,流量是影响柱效和分析时间的关键参数。 活化色谱柱流量的原理主要是基于质量守恒定律,即在稳态下,流速和流量是成正比关系的。一般情况下,流速越快,流量就越大。...
For assessing inhibition of IL-4 and IL-5 secretion in T cells by ABT-281, CD4+ T cells were isolated from PBMC using a T cell subset enrichment column (R&D Systems, Minneapolis, MN). Purified CD4+ T cells were cultured with serially diluted ABT-281 and PMA at 10 ng per ml in ...
Six new cadinane-type sesquiterpenoids, named Chimnitensin A-F (1–6) were isolated from the leaves of Chimonanthus nitens Oliv. Their structures were elucidated by comprehensive spectroscopic analyses and comparison with structurally related known analogues. In vitro MTT assay showed that all six com...
The column was an Acclaim PepMap RSLC C18 75 μm × 15 cm (Thermo Scientific, Waltham, MA, USA) in stationary phase, at a flow rate of 300 nL/min. Mobile phase Solutions A and B consisted of 0.1% formic acid and 80% acetonitrile in 0.1% formic acid, respectively. The initial ...
SP AMI气相色谱分析柱SP column不锈钢填充柱 SP AMI气相色谱分析柱SP column不锈钢填充柱,用于空气和氨气分离气相色谱分析,尺寸为3mX3mm,填料为进口,型号为SP-AMI,测量范围为色谱分析。如果您有需求,该产品可以购买。 产品参数 型号 测量范围 填料 数量 加工定制 SP-AMI 色谱分析 进口 11 否 联系厂家 色谱仪柱 ...