In the 1960s, the column chromatography LC with its low-pressure suitable glass columns was further developed to the HPLC with its high-pressure adapted metal columns. HPLC is thus basically a highly improved form of column liquid chromatography. Instead of a solvent being allowed to drip through...
In recent years, high pressure liquid chromatography (HPLC) has been used for the analysis of triglycerides as well as for other related materials. Specialized gas chromatography columns have been developed for the separation of long-chain fatty acids such as the methyl esters. These columns have ...
Samples for proteotype analysis were prepared using S-trap (Protifi) columns according to the manufacturer’s instructions. For MS analysis, peptides were reconstituted in 5% acetonitrile and 0.1% formic acid containing iRT peptides (Biognosys). Depending on instrument availability and estimated requi...
This study aimed to investigate the sphingolipid characteristics for different types of PCOS using liquid chromatography-mass spectrometry (LC-MS). A total of 107 women with PCOS and 37 healthy women as normal controls were studied. PCOS patients were further classified into non-obesity with insulin...
The sensitivities of MISPME/HPLC–UV analysis for Met-enkephalin and Leu-enkephalin were as well as the results of HPLC–MS/MS combined with SPME [13,14]. Actually, the advantages of HPLC/UV system make it more applicable in the quantization of enkephalins in biological matrices. Show ...
After proteolytic digestion, the samples were quenched using formic acid to a final concentration of 1.0% and subsequently desalted on 10 mg OASIS HLB solid phase columns (Waters). From each condition (n = 8), 50 μg aliquots of the Ng KD dried tryptic peptides were reconstituted in ...
In fact, multisyringe burette can only work under moderate pressures, which lim- its the choice of flow rates. However, its combination with monolithic columns has shown good results, when compared with HPLC, by achieving the required selectivity. The majority of the applications described ...
On the chromatogram with a DEAE-Sephadex column, two peaks of aminopeptidase P were observed and were named APP-I and APP-II. APP-I was further purified by HPLC using DEAE-5PW and Phenyl-5PW columns. Optimum pHs of APP-I and APP-II were 8.0 and 9.0, respectively. In contrast to ...
(Labcyte) at a volume of 47.5 µl per well, resulting in identical plates of up to 352 unique neoepitopes for screening against each of the 15 HLA alleles. The first two columns of the plate were reserved for controls. A*02:01 with and without exchange peptide was included on each...
The extracts were purified using two solid-phase extraction columns, an octadecylsilica-based column (C18, 500 mg of sorbent, Applied Separations) and after that an Oasis MCX column (30 mg/1 mL, Waters, Milford, MA, USA) [43]. Analytes were eluted by a three-step elution using 60% (...