Primary hyperoxaluria type 1 (PH1) is an autosomal recessive inherited disorder resulting from a deficiency in the alanine glyoxylate aminotransferase (AGT) enzyme encoded by the AGXT gene (Danpure et al., 1987; Hopp et al., 2015; Mandrile et al., 2014). Under normal circumstances, AGT detox...
Visum spatial gene expression was processed by using Visum spatial gene expression slide and Reagent Kit (10x Genomics, PN-1000184). Then, 70 µl of permeabilization enzyme was added to the side of each well, and the slide was sealed and placed in a thermocycler adaptor at 37 °C. After...
IL-33-dependent induction of allergic lung inflammation by FcgammaRIII signaling. J Clin Invest. 2013;123:2287–2297. Article CAS PubMed PubMed Central Google Scholar Hardman CS, Panova V, McKenzie AN. IL-33 citrine reporter mice reveal the temporal and spatial expression of IL-33 during ...
(2) Phosphoinositide-dependent kinase-1. P domain See trefoil motif. pds1 (1) In S. cerevisiae, the gene that encodes securin. (2) In Zea mays, the gene that encodes phytoene dehydrogenase, a chloroplast enzyme (EC 1.14.99.-, 571aa) involved in carotenoid biosynthesis. PDX-1 (1) ...
Retinitis Pigmentosa is a genetically heterogeneous, degenerative retinal disorder characterized by gradual dysfunction and death of photoreceptors, first rods and later cones, and progressive blindness. Studies suggested that application of L-type calci
Amyloidogenic processing of APP by β-site APP cleaving enzyme (BACE1) with subsequent cleavage by presenilin-containing γ-secretase, liberates soluble APP beta (sAPPβ) and Aβ3. Aβ monomers can further aggregate into soluble oligomers, protofibrils and large insoluble amyloid fibrils that ...
After attaining the crystallized enzyme, the H2O and inhibitors were removed and the H atoms were added. The examined ligands were redocked into the vacant active site after the standard inhibitor was removed from it. The ChemPLP scoring function was generated for measuring the binding affinity, ...
Baekkeskov S, Aanstoot HJ, Christgau S, Reetz A, Solimena M, Cascalho M, Folli F, Richter-Olesen H, DeCamilli P, Camilli PD: Identification of the 64K autoantigen in insulin-dependent diabetes as the GABA-synthesizing enzyme glutamic acid decarboxylase. Nature. 1990, 347: 151-156. 10.1038...
Repeated hypoglycaemia mostly induced a reduction in gene expression. Among the most downregulated genes wasPcsk1n, an inhibitor of the protein convertase, PCSK1. This enzyme controls the functional maturation of several hormones and neuropeptides, including AVP, and the orexigenic and anorexigenic neur...
The enzyme directly binds to viral dsRNA and is activated to synthesize 2′,5′-linked oligoadenylates from ATP, leading to the dimerization and activation of ribonuclease L (RNase L) (Dong and Silverman, 1995, Malathi et al., 2007). RNase L cleaves cellular and viral ssRNA downstream UU ...