Materials and Methods Plasmid construction The DNA fragments encoding full-length TβRI and EGFR were subcloned into the HindIII and BamHI sites of pEGFP-N1 (Clon- tech), yielding the TβRI-GFP and EGFR-GFP expression plas- mids. The plasmid encoding HA-TβRIII was a gift from Dr ...
Unless otherwise indi- cated, plants were grown under continuous white light at 22 ºC in soil or on Murashige-Skoog (MS) [57] medium (1/2 MS salts, 1% sucrose and 0.8% agar). Plasmid construction and generation of transgenic lines Total RNA prepared from different tissues of wild-...
2.3.1 IBC Merge Mode In IBC merge mode, an index pointing to an entry in the IBC merge candidates list is parsed from the bitstream. The construction of the IBC merge list can be summarized according to the following sequence of steps: Step 1: Derivation of spatial candidates Step 2: Ins...
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The anti-Ubiquitin (P4D1), anti-Myc (9E10), anti-β-Actin and anti-β-Tubulin antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA). Expression vector construction and stable cell lines estab- lishment RNF13 cDNA was cloned into the pcDNA4.0-Myc-His vector with the ...
© 2012 IBCB, SIBS, CAS All rights reserved 1001-0602/12 $ 32.00 www.nature.com/cr npg Phospholipid Scramblase 1 regulates Toll-like receptor 9-mediated type I interferon production in plasmacytoid dendritic cells Amjad H Talukder1, Musheng Bao1, Tae Whan Kim1, Valeria Facchinetti1, ...