These motions highlight differences in flexibility between the two types of protein-protein interfaces and better define the range of conformations available to the catalytically active tetramer. From these results a model implicating steric occlusion in the enforcement of half-of-the-sites activity is...
However, the number of possible conformations of the polypeptide backbone is so large that random sampling would n... S Linse,Bjoern Linse - 《Journal of the American Chemical Society》 被引量: 26发表: 2007年 A new dataset of non-redundant protein/protein interfaces Here we address the ...
dual conformationshuman TrpRStRNAAminoacylation of tRNA is the first step of protein synthesis. Here, we report the co-crystal structure of human tryptophanyl-tRNA synthetase and tRNATrp. This enzyme is reported to interact directly with elongation factor 1, which carries charged tRNA to the ...
The conformations of filamentous and soluble tau associated with Alzheimer paired helical filaments. Paired helical filaments (PHF) occur in Alzheimer's diseased brains and are known to be composed of the microtubule-associated protein, tau. In the present... Goux,J Warren - 《Biochemistry》 被引...
Sex hormone-binding globulin (SHBG) is the major sex steroid-binding protein in human plasma and is produced by the liver. Plasma SHBG levels vary consider... KN Hogeveen,M Talikka,GL Hammond - 《Journal of Biological Chemistry》 被引量: 232发表: 2001年 MSH2 and ATR form a signaling mod...
Advances in protein design have brought us within reach of developing a nanoscale programming language, in which molecules serve as operands and their conformational states function as logic gates with precise input and output behaviors. Combining these
In the structure of SIN3L, each Sin3 isoform (Pst1 and Pst3) interacts with one histone deacetylase Clr6, and one WD40-containing protein Prw1, forming two lobes. These two lobes are bridged by two vertical coiled-coil domains from Sds3/Dep1 and Rxt2/Png2, respectively. In the ...
The inhibitor of chromosomal replication (iciA) protein encoded by M. tuberculosis has been suggested to inhibit chromosome replication initiation in vitro. However, iciA has also been identified as arginine permease (ArgP), a regulatory transcription factor for arginine outward transport. In order to...
Cas9, the signature protein of the type II system, cleaves the target and non-target strands by its HNH and RuvC catalytic domain. In contrast, Cas12a, the hallmark protein of the type V system, uses a single RuvC domain for cleavage of both strands of target DNA. Cas9 and Cas12a are...
However, the search for stable local conformations has focused mainly on regular secondary structural elements [2]. The truncated 64-residue form of the small monomeric protein chymotrypsin inhibitor 2 (CI2), lacking the first, unstructured, 19 amino acids, is a very good model system for ...