Processive DNA synthesisReplisomeBackground DNA polymerase D (PolD) is the representative member of the D family of DNA polymerases. It is an archaea-specific DNA polymerase required for replication and unrelated to other known DNA polymerases. PolD consists of a heterodimer of two subunits, DP1 ...
2014. Parallel DNA polymerase chain reaction: Synthesis of two different PCR products from a DNA template. F1000Research. 3: 320... V Bhardwaj,K Sharma - 《F1000res》 被引量: 2发表: 2015年 The use of a specific DNA probe and polymerase chain reaction for the detection of Mycobacterium ...
amplified in 50 μl of reaction mixture containing 0.3 μM of each primer, 200 μM of each dNTP (Pharmacia), 5 μl of 10 × reaction buffer (100 mM Tris-HCL [pH 9.0], 500 mM KCL, and 1% Triton X-100), 5% dimethyl sulfoxide, 1.5 mM MgCl2, and 1.25 units Taq DNA polymerase. ...
本试剂盒含有从RNA到cDNA,以及qPCR扩增cDNA的全部试剂。2×Green qPCR MasterMix是专用于染料法(SYBR Green I)实时荧光定量PCR的预混体系,包含HotStar Taq DNA Polymerase、PCR Buffer、dNTPs、SYBR Green I荧光染料、Mg2+和ROX校正染料,操作简单方便,主要用于基因组DNA靶序列和RNA反转录后cDNA靶序列的检测。
Two novel DNA motifs are essential for BACE1 gene transcription Yan Xiang1*, Shasha Meng1*, Jinfeng Wang1, Songyang Li1, Jingru Liu1, Hongmei Li1, Tingyu Li1, Weihong Song1,2 & Weihui Zhou1 1Ministry of Education Key Laboratory of Child Development and Disorders; Chongqing Key Laboratory...
基因扩增技术(PCR)聚合酶链反应(polymerase chain reaction简称PCR)又称无细胞分子克隆系统或特异性DNA序列体外引物定向酶促扩增法,是基因扩增技术的一次重大革新。 PCR扩增DNA的原理是:先将含有所需扩增分析序列的靶DNA双链经热变性处理解开为两个寡聚核苷酸单链,然后加入一对根据已知DNA序列由人工合成的与所...
Using transient inhibition of DNA mismatch repair during a permissive stage of development, we demonstrate highly efficient prime editing of mouse embryos with few unwanted, local byproducts (average 58% precise edit frequency, 0.5% on-target error frequ
Structural basis for the 3′-5′ exonuclease activity of Escherichia coli DNA polymerase I: A two metal ion mechanism The refined crystal structures of the large proteolytic fragment (Klenow fragment) of Escherichia coli DNA polymerase I and its complexes with a deoxynucle... L.S. Beese,T.A...
Fig. 10: A working model on the functions of REN and RopGAP in coordinating tip growth and cell width regulation. REN has a higher GAP activity but a weaker binding capacity to active ROPs than RopGAPs. During polarity establishment, RopGEFs convert ROP-GDP (inactive) to ROP-GTP (active)...
A maternally transmitted factor, Mtf determines the antigenic polymorphism of the major histocompatibility complex (MHC) class I-like maternally transmitted antigen (Mta) (1, 2). Most inbred strains of mice express Mtaa (MY"), while NZB/BlNJ is Mta b+ (Mtf +). NZB mitochondrial DNA (mtDNA...