DNA (deoxyribonucleic acid) polymerase refers to a group of enzymes involved in DNA replication. These enzymes add mononucleotides to the 3' end of a DNA strand in the replication fork. There are mainly three types of DNA polymerases, including DNA Pol I, II, and III.Answer and...
DNA Polymerases: DNA polymerases are a group of enzymes which catalyze the formation of phosphodiester bonds between phosphate groups and ribose sugars. They are thus essential for DNA replication. Polymersase Chain Reaction (PCR): The Tas DNA polymerase is widely used in PCR, and has been since...
In contrast, primers are specifically designed to work with DNA polymerases, initiating the synthesis of a new DNA strand complementary to the target DNA sequence. 12 While probes provide real-time data and can be used in living cells to study dynamic biological processes, primers are typically...
Most DNA polymerases that are used for PCR work best at temperatures around 68°C to 72°C. The enzyme can, however, also be active at lower temperatures, although to a lower degree. At temperatures far below the annealing temperature, primers can bind non-specifically and lead to non-...
RNA transcription is associated with a transcription bubble, which consists of an unwound template DNA that is accessible to RNA polymerases and transcription factors.The short answer to your second question is no, transcription generally does not happen at the same time as DNA replication. In ...
Overview of the PCR procedure A typical PCR procedure can be broken down into different steps. These are described in more detail below. 1. Initial denaturation This step is performed for two reasons. The first is to activate the Taq polymerase enzyme. The second is to denature the DNA start...
Bacteria are unicellular i.e. made up of a single cell. They are prokaryotes and their cells are different from animal and plant cells. In general, the structure of bacteria can be studied as external and internal structures; Structure of a Bacterial Cell ...
>> Adam Lauring: So, at its basic, it means its genome is made of RNA and not DNA. But functionally, what it means is RNA viruses tend to have higher mutation rates than DNA viruses, and that's because the enzymes, the polymerases that the virus uses to copy its genome, tend to ...
There is considerable similarity between all published genetic sequences for the SARS-CoV-2 virus in humans, which suggests that the outbreak began with the virus moving into the human population at one point, in time that first reports appeared. However, researchers do not currently know the ...
How are the 3' and 5' of a DNA molecule different and which end grow during replication? What is the difference between a template and a primer in DNA replication? What is the difference between DNA replication, transcription, and translation? Do they all work together?