现在,宾夕法尼亚州立大学的研究人员开发了一种新方法,可以在一次实验中测试数千种预测核酶的活性。 研究小组测试了2600多种不同的RNA序列的活性,这些序列被预测属于一类被称为“扭曲核酶(twister ribozymes)”的RNA酶,这种酶有能力将自己切成两半。大约94%的测试核酶是活跃的,研究表明,即使它们的结构有轻微的缺陷,...
We report on the 2.9- crystal structure of thetwister ribozyme, which adopts a compact tertiary fold stabilized by co-helical stacking, double-pseudoknot formation and long-range pairing interactions. The U-A cleavage site adopts a splayed-apart conformation with the modelled 2′-O of U ...
2021年9月22日,我院Alastair Murchie-陈东戎课题组在Nucleic Acids Research发表题为“The function of twister ribozyme variants in non-LTR retrotransposition in Schistosoma mansoni”的研究成果,报道了Twister核酶参与S.mansoni基因组中perere-3 non-LTR逆转座的生物学功能。 Alastair Murchie-陈东戎课题组运用RN...
The utilization of ribozyme-based synthetic switches in biotechnology has many advantages such as an increased robustness due to in cis regulation, small coding space and a high degree of modularity. The report of small endonucleolytic twister ribozymes
2017年10月30日,任艾明课题组与奥地利因斯布鲁克大学Ronald Micura教授课题组合作在Nature Communications发表题为"Structure-based Insights into Self-Cleavage by a Four-way Junctional Twister-Sister Ribozyme"的研究论文。任艾明课题组2016级直...
Figure 1. Structure of Twister ribozyme and spatially adjacent phosphate pairs. (a) Twister Ribozyme secondary and tertiary contacts are shown. The scissile bond is indicated with a solid arrow, and the omitted P3 and P5 loops are indicated. (b) Contacts are illustrated as cartoons colored as ...
ozyme Catalysis']]>Messina, Kyle J.Bevilacqua, Philip C.Journal of the American Chemical Society
Crystal structure and mechanistic investigation of the twister ribozyme. We present a crystal structure at 2.3- resolution of the recently described nucleolytic ribozyme twister. The RNA adopts a previously uncharacterized compa... Y Liu,TJ Wilson,SA Mcphee,... - 《Nature Chemical Biology》 被引量...
The twister ribozyme is a widely distributed selfヽleaving RNA enzyme. The aminoglycoside antibiotics are a clinically important class of flexible RNA‐binding ligands. Aminoglycoside binding can modulate ribozyme activity such that paromomycin inhibits and sisomicin activates the ribozyme. Drug binding ...
All of the single and double mutants of a twister ribozyme (a total of 10 296 mutants) were generated and assayed for their self-cleaving activity by exploiting deep sequencing to count the numbers of cleaved and uncleaved sequences for every mutant. Interestingly, we found that the ribozyme ...