小的自剪切型核酶(small self-cleaving ribozyme)发现于30多年前,长度在50到150 个碱基之间,至今只有九种该类核酶被报道。Twister-sister 是于2015年被发现的一种新型核酶。 利用x-射线晶体学的方法,任艾明课题组首次解析了完整的四通道的...
We have undertaken cleavage assays in the context of a two-stranded construct of the twister-sister ribozyme (Fig.4a; Supplementary Fig.1a). Cleavage data on the wild-type twister-sister ribozyme under 2 mM Mg2+, Mn2+, Ca2+, and Sr2+divalent cation conditions are compared in Supplementary...
These two self-cleaving ribozymes, which are shaped like lanterns, are similar to the catalytic regions of the twister sister ribozymes in terms of sequence and secondary structure. However, the nucleotides at the cleavage site have shown that mutational effects on two twister sister...
Recently, new widespread classes of self-cleaving ribozymes called twister, twister sister, pistol and hatchet were discovered using a bioinformatics pipeline2,3. Meanwhile, crystal structures of twister ribozymes have revealed a highly compact fold comprising two pseudoknots and have provided new ...