32 (12), 1276-81.Cameron DE, Collins JJ. 2014. Tunable protein degradation in bacteria. Nat Biotechnol 32:1276 -1281. http://dx.doi.org/10.1038/nbt.3053.Cameron DE, Collins JJ (2014) Tunable protein degradation
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Protein expression in bacteria and purification Plasmids were transformed in BL21 (DE3) competent Escherichia coli (New England Biolabs) or Rosetta (DE3) pLysS E. coli (Merck). Cells were grown at 37 °C in a lysogen broth medium supplemented with 50 μg.mL–1 kanamycin (and 34 μ...
Predictable tuning of protein expression in bacteria. Nat Methods. 2016;13:233–6. 16. Elf J, Li G, Xie XS. Probing transcription factor dynamics at the single-molecule level in a living cell. Science. 2004;316:1191–4. Martyushenko et al. BMC Systems Biology (2016) 10:39 Page 14 ...
Bacteria used in this study include Staphylococcus aureus USA300 (methicillin-resistant), Staphylococcus epidermidis ATCC49134, Bacillus subtilis BR-151, Escherichia coli JM109, Pseudomonas aeruginosa ATCC9027 (intermediate resistance to carbenicillin and piperacillin), and 9 clinically isolated pathogens from...
2014. Tunable protein degradation in bacteria. Nat Biotechnol 32:1276 -1281. http://dx.doi.org/10.1038/nbt.3053.D. E. Cameron, J. J. Collins, Tunable protein degradation in bacteria. Nat. Biotechnol. 32, 1276-1281 (2014)....
The methods and compositions described herein relate, in part, to the generation of a synthetic degradation system in that provides tunable control of the protein level of targeted genes by using components of the tmRNA system. Provided herein are degradation tag variants that permit independent ...
The methods and compositions described herein relate, in part, to the generation of a synthetic degradation system in E. coli that provides tunable control of the protein level of targeted genes by using components of the Mesoplasma florum tmRNA system. Provided herein are degradation tag variants ...
The present invention provides a means to modulate gene expression in vivo in a manner that avoids problems associated with CRISPR endogenous protein knock-out or knock-in strategies and strategies that provide for correction, or alteration, of single nucleotides. The invention includes inserting into...
The last decade has seen bacteria at the forefront of biotechnological innovation, with applications including biomolecular computing, living therapeutics, microbiome engineering and microbial factories. These emerging applications are all united by the