Click on the tumor marker to go to the test article for more details on a particular marker. Tumor markerAssociated cancer(s)Usual sampleUse(s)Comments AFP (Alpha-feto protein) Certain liver, ovarian, testicular Blood Helps diagnose, monitors treatment and for recurrence Also elevated during ...
Differentially expressed genes in each cluster were identified using the FindAllMarker() function built within Seurat package and a corresponding p-value was given by the Wilcoxon’s test followed by a Bonferroni correction. Top differentially expressed gene markers were illustrated in a stacked violin...
All flow cytometry experiments included live/dead staining to discriminate live or dead cells and we applied Fluorescence Minus One control to define positivity of every marker of interest. Flow cytometry data were acquired on an CytoFLEX LX Flow Cytometer with CytExpert v2.3 software and we used ...
(MECA-79, BioLegend), Anti-Human/Mouse High Endothelial Venule Marker-Alexa Fluor 488 (Clone MECA-79, eBioscience), gp38/Podoplanin-biotin (eBio8.1.1, eBioscience), gp38/Podoplanin-PeCy7 (eBio8.1.1, eBioscience), PD-L1-PeCy7 (10F.9G2, BioLegend), IFNγ-Alexa Fluor 647 (XMG1.2...
Together, our results reveal a novel protumoral role of ectosomal PKM2 in HCC progression, which could serve as a promising clinical diagnostic marker and therapeutic target. Results HCC-Cell-Derived Ectosomal PKM2 Promotes Monocyte-to-Macrophage Differentiation Exosomes (indicated as Exo) and ...
fibroblasts showed high expression of chemokines (CCL11, CXCL12, and CXCL14) (Fig.2b; Table S4), similar to the previously reported iCAFs in various types of tumors such as BC [6] and PTC [8]. GO enrichment analysis of its marker genes showed their association with the response to ...
Conclusion Tumor suppressor protein SMAR1 might be used as a phenotypic differentiation marker between cancerous and non-cancerous cells.Peer Review reports Background Even though the current diagnosis of cancer depends mainly upon tissue biopsy inspection by routine optical microscopy [1], imaging tool...
A bead-based microarray for exosome isolation and multiplexed tumor marker detection was developed. The beads are uniformly trapped and queued among the micropillars in the chip, which can avoid optical interference and enable more accurate test results. The results with different types of lung cance...
2J). These results emphasize the heterogeneity of malignant epithelial cells in PDAC and suggest that single-marker analysis may be insufficient to accurately identify cancer cell subtypes within a patient’s tumor. Charting the CAF landscape in the PDAC tumor microenvironment We next analyzed the ...
Tissue types of each cluster were inferred and clusters were further refined by plotting clusters onto the associated histology images and identifying marker genes using the Wilcoxon’s Rank Sum test. Expression scores for ETS and cilia gene sets were calculated using the Seurat function AddModule...