Small molecule inhibitors of TSLP signaling pathways are also under development and hold promise as oral therapies for long-term disease management. Combination therapies targeting multiple inflammatory pathways, including TSLP, may offer synergistic effects and improve treatment outcomes in severe asthma ...
baicalensis, as the first small molecule inhibitor of the hTSLP signaling pathway. In vitro studies including ELISA, flow cytometry, and Western-blot analysis showed that 1 blocked the interaction between hTSLP and hTSLPR in a dose-dependent manner. The HDX-MS experiment also confirmed that 1 ...
Thymic stromal lymphopoietin (TSLP) plays an important role in the differentiation and proliferation of Th2 cells, resulting in eosinophilic inflammation and numerous allergic diseases. Baicalein (), a major component of, was found to be the first small molecule to block TSLP signaling pathways. It...
For western blotting, cells were harvested by scraping in Iscove's modified Dulbecco's medium (Sigma-Aldrich) containing 10% FBS, centrifuged, and washed once in ice-cold PBS before lysis with 0.5% NP-40 (Millipore, Merck Chemicals, Nottingham, UK) in PBS supplemented with protease inhibitor ...
Src-type mediators are also involved in proliferative signaling, TSLP-induced cell proliferation is blocked by the Src family inhibitor PP1 [34]. While both STAT5 activation and cell proliferation require the box1 domain of TSLPRα and IL-7Rα, the single intracellular tyrosine residue of TS...
(DSS)-mediated epithelial insult11 or in a model of CD4 þ T-cell transfer colitis.10 Furthermore, TSLP is critical in mediating mucosal healing after DSS insult through its ability to stimulate secretory leukocyte peptidase inhibitor expression by intestinal epithelial cells (IECs).24 We ...
Protein samples for NMR measurements at concentrations of 582 mM (8.5 mg ml À 1) for 15N-TSLPD127–131 and 628 mM (9.2 mg ml À 1) for 13C/15N-TSLPD127–131 were prepared in 20 mM NaH2PO4 pH 6.8, 50 mM NaCl, 2.5 mM EDTA and 1 Â cOmplete Protease Inhibitor cocktail. ...
Atopic dermatitis (AD) is one of the most common chronic inflammatory skin diseases worldwide, characterized by intense pruritus and eczematous lesions. Aberrant expression of thymic stromal lymphopoietin (TSLP) in keratinocytes is associated with the pa
TSLP downregulation by an MDM2 inhibitor, nutlin-3a, alleviated clinical symptoms and septic inflammatory responses. Pharmacological inhibition of TSLP level by cisplatin reduced the septic inflammatory responses. Altogether, the present results show that TSLP exacerbates septic inflammation via the MDM2 ...