S-(2-Ethylhexyloxycarbonylmethyl)- l-cysteine was synthesized from l-serine and 2-ethylhexylthioglycolate using intact cells of recombinant E. coli with high tryptophan synthase activity, and subsequently hydrolyzed to l-CMC with alkali in one reactor. Under optimum conditions established for l-CMC...
The α2β2 complex of tryptophan synthase from Escherichia coli catalyzes β-replacement reactions of l-serine and its derivatives (e.g., β-chloro-l-alanine and O-methyl-Dl-serine) with various alkanethiols. The products from thiobenzyl alcohol and ethanethiol were isolated to demonstrate the...
SELD EDP08654 Selenophosphate synthase (SelD) 2.7.9.3 SPCS EDP02915 Selenocysteine synthase; Sep-tRNA:Sec-tRNA synthase (SepSecS) 2.9.1.1 In higher plants, the two enzymes involved in cysteine synthesis are found in plastids, mitochondria, and the cytosol (Leustek et al., 2000) and the same...
Disruption of trpE, which encodes anthranilate synthase component I, was chosen because four genes of the tryptophan operon were highly up-regulated during biofilm growth and the product of trpE catalyses the first reaction of the tryptophan pathway with TrpD (Table 1). Moreover, TrpE is the ...
Likewise, indole acetaldehyde synthase depends on l-tryptophan supply (Fig. 1). In this study, we present a transcriptomic analysis of P. mexicana with particular emphasis on genes involved in the l-tryptophan meta-bolism. We investigated how the genes of the tryptophan branch of the shikimate...
et al. A Projector-Embedding Approach for Multiscale Coupled-Cluster Calculations Applied to Citrate Synthase. Chem. Theory Comput. 12, 2689–2697 (2016). Article CAS Google Scholar Soto-Delgado, J., Tapia, A. R. & Torras, J. Multiscale Treatment for the Molecular Mechanism of a Diels–...
(4k) reacted with maleimide to give product5k’via the Michael addition reaction. The peptide4lcontaining cysteine failed to yield the desired product most probably due to the presence of the easily oxidized sulfhydryl group. Fig. 3: Substrate scope for maleimidation of dipeptides and complex ...
(L. reuteri), is sufficient to exacerbate EAE pathogenesis in mice [86,87]. Moreover, we found that the presence ofL. reuteriwas associated with altered levels of circulating tryptophan metabolites [87]. Here, we leveraged whole genome sequencing of commensalL. reuteriisolates, which revealed ...
However, it has been reported that various factors are believed to be involved in the mechanisms of MSC-mediated immunosuppression, including inducible nitric oxide synthase (iNOS), IDO, tumor necrosis factor-inducible gene 6 (TSG6), CC-chemokine ligand 2 (CCL2), IL-10, and prostaglandin E2 ...
Maize also contains a tryptophan synthaseα-like protein, unlinked to a tryptophan synthase β protein, that produces free indole [23]. Thus, there exists the potential for fine-tuning the regulation of this pathway by differential regulation of the expression of these genes, or differential ...