pallens adults were significantly affected when boric acid was mixed with the honey–water solution as a positive control. The uptake, temporal stability and bioactivity of Cry1Fa and Vip3Aa before and after C. pallens access to honey–water were confirmed using double antibody sandwich enzyme-...
Proteins differ from peptides in that they cannot be effectively resolved by time of flight MS and they are too large (>3 kD) to be effectively fragmented by collision with gases. The most commonly used solution to these problems is to resolve the proteins by polyacrylamide gel electrophoresis...
In duplicate, the flour samples were extracted using a sodium acetate buffer solution with a pH of 3.8 and ionic strength of 0.02 N as reported by Chang and Tsen [28]. Morever, 1 g of each flour sample was homogenized with 15 mL of solvent using an automatic shaker at 160 rpm for 1...
The microwaved tissue on the glass slide was cooled to room temperature for 20 min and treated with 5% goat serum, a blocking solution. Next, the tissue was washed three times with 1X PBS for 5 min. Caspase-1 antibody (Invitrogen, Waltham, MA, USA) was used as the primary antibody ...
Several studies have sought new therapies for obesity and liver diseases. This study investigated the effect of the trypsin inhibitor isolated from tamarind seeds (TTI), nanoencapsulated in chitosan and whey protein isolate (ECW), on the liver health sta