In practical terms, our results suggest that only peptides originating from specific trypsin cleavage be considered in database searches. In order to identify as many peptides as possible, several changes to database search algorithms could, however, be considered. First, peptides with many interna...
Fig. 5. MS/MS fragmentation patterns recorded at the 20% HCD level from a disulfated and sialylated bikunin CS-glycopeptide precursor ion. A, The complete HCD spectrum of the [M+3H]3+ precursor ion at m/z 1191.46. B, The complete HCD spectrum of the [M+3Na]3+ precursor ion at m...
As the reference genes play a major role in normalizing the expression level of the gene of interest in the gene expression profiling, the most expression stable reference gene need to be validated. Once the efficiencies and specificity of the primers were confirmed, validation of the most ...