Site-directedmutagenesistroubleshootingcontrolsBy shifting pedagogical goals from obtaining successful mutations to teaching students critical troubleshooting skills, it has been possible to introduce site-directed mutagenesis into an undergraduate teaching laboratory. Described in this study is an inexpensive ...
Suggested approaches here involve co-localization studies and site-specific mutagenesis giving rise to mutants that perturb the binding process. What can I do if little or no tagged protein appears on my western blot af...