Simplicity—free online toolguides you through construct and primer design and generates final construct sequence for easy implementation Which site-directed mutagenesis system is right for you? GeneArt Site-DirectedMutagenesis SystemGeneArt Site-Directed Mutagenesis PLUS System ...
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Site-directed mutagenesis is a powerful research tool used to study protein function, identify enzyme active sites, and design novel proteins in drug discovery.
Site-directed mutagenesis (SDM) aims to introduce precise alterations in any coding or noncoding deoxyribonucleic acid (DNA) sequence, usually in vitro . These modifications could be as small as a nucleotide or several hundred; in one site or in multisite in the same DNA sequence. Recently, ...
Rational design by site-directedmutagenesishas enjoyed some success in the past, mostly in term of engineeringenzyme specificity. Our ability to redesignenzyme mechanismsor completely new reactions, however, remains a difficult task. With the ever increasing knowledge of protein structure and function,si...
PCR site-directed mutagenesis is one of the most often-used techniques for introducing a mutation into a DNA sequence, however most software tools don’t consider the impact of a mutation on protein structure when designing primers. Lasergene provides a unique approach to primer design for PCR ...
1.Site-directed mutagenesis of functional gene fragment producing CTX-M-14-type extended-spectrum β-lactamase;CTX-M-14型超广谱β-内酰胺酶基因的定点突变研究 2.Study on site-directed mutagenesis and kinetics of trehalose synthase from Thermobifida fusca;Thermobifida fusca海藻糖合成酶的定点突变及其动力...
5) Site directed mutagenesis 定点突变 1. Using recombinant glucagon expression system,the 21st aspartic acid was changed to be alanine by means of site directed mutagenesis. 在获得重组胰高血糖素基因工程菌基础上 ,利用定点突变技术改造其第 2 1位氨基酸天冬氨酸为丙氨酸 ,并经DNA测序证明胰高血糖素...
1. Site-directed mutagenesis of functional gene fragment producing CTX-M-14-type extended-spectrum β-lactamase; CTX-M-14型超广谱β-内酰胺酶基因的定点突变研究2. Study on site-directed mutagenesis and kinetics of trehalose synthase from Thermobifida fusca; Thermobifida fusca海藻糖合成酶的定点突变...
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