It is important to keep all these items in mind when troubleshooting HPLC problems. Knowing where to start and what to do will be discussed in the next several topics. Peak Broadening As chromatographers, we are constantly trying to get sharp, efficient peaks and baseline resolution for our ...
4. Isolate the Pump Problems The HPLC pump is mainly responsible for delivering the mobile phase at a precise flow rate. If you are experiencing flow rate deviations or any irregularities, you can check for air bubbles in the pump’s inlet line and degas the solvent if it is necessary. Al...
The chapter also summarizes common HPLC troubleshooting and diagnostic strategies. Discussion focuses on common problems, their associated symptoms, and typical remedial actions. The chapter presents several case studies concerning baseline and data performance problems, as well as troubleshooting examples, ...
7. Buffers and Baselines 8. Communicating with the Baseline 9. When Ideal Isn’t Practical 10. Attacking Carryover Problems 11. Retaining Polar Compounds 12. Ion Suppression in LC-MS-MS --- A Case Study 13. Quiet, Please 14. Stability-Indicating Assays 15. Peak Tailing and Resolution 16....
8. Communicating with the Baseline 9. When Ideal Isn’t Practical 10. Attacking Carryover Problems 11. Retaining Polar Compounds 12. Ion Suppression in LC-MS-MS --- A Case Study 13. Quiet, Please 14. Stability-Indicating Assays 15. Peak Tailing and Resolution 16...
GPC/SEC Troubleshooting Guide Your guide to solving common problems and staying productive Places to Start Solvents – Always choose HPLC-grade solvents and filter through 0.02–0.45 μm filters – Use stabilized solvent when appropriate (e.g., THF stabilized with butyl hydroxylated toluene (BHT) ...
Data acquisition mode — Full scan or SIR — Centroid, continuum or MCA ©2007 Waters Corporation 36 HPLC Split Waste Probe Analytical Column MS/MS LC/MS System Setup LC/MS System Setup PDA / UV ©2007 Waters Corporation 37 Electrospray Electrospray Ionization Ionization Sample ion in ...