Baseline Noise 21. Narrow-bore Columns 22. Sample Solvent 23. Gradient Scaling 24. Column Storage 25. Paired-Ion Chromatography 26. Hydrolytic Stability of Reversed-Phase Packings 27. Optimal Flow Rates 28. Carbon Load 29. pH Control 30. Mobile Phase Composition 31. Column Contamination 32. ...
11. Column Plate-Count 12. Column Backpressure 13. Peak Area Fluctuations 14. Ghost Peaks 15. Dependence of Retention Times on pH 16. Column Equilibration 17. Column Conditioning 18. Complex Sample Matrices 19. Hydrophobic Collapse 20. Baseline Noise 21. Narrow-bore Columns 22. Sample Solvent 2...
Baseline noise has a significant contribution to the repeatability of chromatographic measurements. In this study, baseline noise is characterized by calculating its power spectrum and wavelet transform using frontal chromatograms. Wavelet analysis is a very powerful means to detect the heteroscedastity ...
5 Identifying Chromatographic Problems 76 Table 5-3 Correcting Chromatographic Problems (Continued) Symptom Possible Cause Corrective Action Baseline noise, random Eluents not properly degassed Degas eluents. Flow erratic, pump not primed Prime the pump (see Section 3.2.1, Priming and Purging the Pump...
In HPLC analysis, water is essential for use in preparing mobile phases and samples. If impurities are present in a mobile phase, however, they can increase background signals that can increase baseline noise, drift, or cause other phenomena, such as negative peaks or unknown peaks during ...
Baseline noise ↑↓ Spectral resolution ↑↓ Bandwidth ↑↓ S/N ratio ↑↓ Spectral resolution ↓↑ 36 Effects of Slit Width Slit width - 1nm 4 x light > 0.5 x noise 37 Effects of Slit Width Slit width - 16nm 4 x light > 0.5 x noise 38 Setting Bandwidth Bandwidth 4 nm s/n = 5...
Replace the deuterium lamp if the baseline noise and/or drift exceed your application limits or if the lamp does not light. To replace the lamp, turn the lamp off and allow it to cool for at least five minutes. Remove the front cover. Unscrew the lamp cover and remove it. Disconnect ...
HPLC Troubleshooting Symptom Type Baseline disturbance at void time Detector leaks Drifting baseline Ghost peaks High column backpressure Possible Cause Positive/negative—Difference in refractive index of injection solvent Plugged inlet frit Positive direction—Contaminant buildup/elution Positive/negative—...
MS/MS provides the solution to many of these problems. However, we believe that information about peptide retention times derived from HPLC-MS measurements could become an important additional tool for MS-based identification. Table IV demonstrates a number of advantages of HPLC-MS and of our ...
Simultaneous analysis of these five compounds with HPLC-ultraviolet detector showed great drift and significant baseline noise the impact of which on the precision and accuracy is unacceptable. Although HPLC or gas chromatography coupled with mass spectrometry is able to quantify these compounds [19,20...