[3]Ningning Shi et al. Restoration of dystrophin expression in mice by suppressing a nonsense mutation through the incorporation of unnatural amino acids. Nature Biomedical Engineering (2021). [4]hC Bioscience Announces $24 MillionSeries A Financin...
The resulting misacylated tRNAs are then converted to cognate pairs through transformation of the amino acids on the tRNA, which is catalyzed by a group of tRNA-dependent modifying enzymes, such as tRNA-dependent amidotransferases, Sep-tRNA:Cys-tRNA synthase, O -phosphoseryl-tRNA kinase and Sep...
人类MTO1(hMTO1)是高度保守的mt-tRNA修饰酶,根据超嗜热菌(Aquifex aeolicus)MnmG的晶体结构[50],hMTO1可能在修饰τm5U过程中结合FAD,负责与tRNA结合,但这些都需要进一步验证。在酵母中敲除MTO1基因,会导致线粒体呼吸和翻译受损,而...
4. Mom Das, et al. Distinct tRNA recognition strategies used by a homologous family of editing domains prevent mistranslation. Nucleic Acids Res. 2014 Apr; 42(6): 3943–3953.5. Kyle Mohler, et al. Translational fidelity and mistranslation in the cellular response to stress. Nat Microbiol. ...
An evolved pyrrolysyl-tRNA synthetase with polysubstrate specificity expands the toolbox for engineering enzymes with incorporation of noncanonical amino acids. 引用方式: Liu, K., Jiang, L., Ma, S., Song, Z.D., Wang, L., Zhang, F.Q., Xu, R.H., Yang, L.R., Wu, J.P., Yu, ...
7. Smolskaya S, et al. Site-Specific Incorporation of Unnatural Amino Acids into Escherichia coli Recombinant Protein: Methodology Development and Recent Achievement. Biomolecules. 2019 Jun 28;9(7):255. 8. Li Li, et al. Naturally occurring aminoacyl-tRNA synthetases editing-domain mutations that ...
7. G. X. Peng et al., RNA granule-clustered mitochondrial aminoacyl-tRNA synthetases form multiple complexes with the potential to fine-tune tRNA aminoacylation. Nucleic Acids Res, 50, 12951-12968 (2022).8. N. M. Reynolds et al., Cell-specific differences in the requirements for translation...
The main role of tRNAs is to carry amino acids into ribosomes for protein synthesis under the guidance of mRNA. During biogenesis, tRNAs can be specifically cleaved into many small fragments by several enzymes. tsRNAs are mainly classified into tRNA-derived fragments (tRFs) and tRNA halves. Howe...
M. Evolutionary tuning impacts the design of bacterial tRNAs for the incorporation of unnatural amino acids by ribosomes. Curr. Opin. Chem. Biol. 46, 138–145 (2018). CAS PubMed PubMed Central Google Scholar LaRiviere, F. J., Wolfson, A. D. & Uhlenbeck, O. C. Uniform binding of ...
举例而言,非天然氨基酸通过微量注射经化学错误酰化的嗜热四膜虫(Tetrahymena thermophila)tRNA(例如,M.E.Saks等人,(1996),An engineered Tetrahymena tRNAGln for in vivo incorporation of unnatural aminoacids into proteins by nonsense suppression,J.Biol.Chem.27123169-23175)和相关mRNA而引入爪蟾卵母细胞(Xenopus ...