在神经细胞中所有其他的tRNA基因的RPC1占位都显著降低,而tRNA-Arg-TCT-4-1基因表达上调,这可以代表一类罕见的强选择性tRNA表达案例。人类tRNA-Arg-TCT-4-1基因与其他的tRNA基因距离都大于2.25Mbp,而离CADM3的转录起始位点只有30kbp,他们发...
4 Orellana, E. A.et al.METTL1-mediated m(7)G modification of Arg-TCT tRNA drives oncogenic transformation.Mol Cell, doi:10.1016/j.molcel.2021.06.031 (2021). 5 Dai, Z.et al.N(7)-Methylguanosine tRNA modification...
从机制上讲,过表达METTL1导联可增加m7G水平,并增加tRNA亚群的丰度。令人惊讶的是,这些丰度增加了的tRNA之一是中枢神经系统特异性tRNA-Arg(反密码子TCT) 4-1。过表达该tRNA部分再现了METTL1过表达时观察到的恶性转化,因此支持了观点:tRNA池的变化和密码子偏倚的mRNA翻译是这种恶性表型的原因。 5 被改变的tRNA成熟和...
Accordingly, Arg-TCT expression is elevated in many tumor types and is associated with patient survival, and strikingly, overexpression of this individual tRNA induces oncogenic transformation. Thus, METTL1-mediated tRNA modification drives oncogenic transformation through a remodeling of the mRNA "transla...
在神经细胞中所有其他的tRNA基因的RPC1占位都显著降低,而tRNA-Arg-TCT-4-1基因表达上调,这可以代表一类罕见的强选择性tRNA表达案例。人类tRNA-Arg-TCT-4-1基因与其他的tRNA基因距离都大于2.25Mbp,而离CADM3的转录起始位点只有30kbp,他们发现在神经细胞中tRNA-Arg-TCT-4-1和CADM3是被共同调控的。MAF1是Pol III...
METTL1 changes ArgTCT4-1, a tRNA that reads the AGA codon, leading to the promotion of the translation of cellular oncogenes in these tumors [4]. This indicates that the effective transcription of tRNAs, which have high levels of methylation modification, boosts the synergistic translation of...
function of METTL1/WDR4 enhances a subset of tRNAs, including the isodecoder Arg-TCT-4–1, leading to malignant transformationthrough enhanced translation of cell-cycle progression mRNAs. Overexpression of m7G-tRNA Arg-TCT-4–1 can mimic the effects of METTL1/WDR4 overexpression (Orellana et ...
Recently, Orellana et al revealed that the METTL1/WDR4 complex could regulate the oncogenesis of many types of human cancers, such as breast cancers, glioblastomas, certain sarcomas, and AML via tRNA ArgTCT−4–1 mediated translation bias of cell growth associated mRNAs, and emphasis on the...
A. et al. METTL1-mediated m(7)G modification of Arg-TCT tRNA drives oncogenic transformation. Mol. Cell 81, 3323–3338.e3314 (2021). Article CAS PubMed PubMed Central Google Scholar Liu, H. et al. Targeting tumour-intrinsic N(7)-methylguanosine tRNA modification inhibits MDSC recruitment...
A. et al. METTL1-mediated m(7)G modification of Arg-TCT tRNA drives oncogenic transformation. Mol. Cell 81, 3323–3338.e14 (2021). Article CAS PubMed PubMed Central Google Scholar Han, H. et al. N(7)-methylguanosine tRNA modification promotes esophageal squamous cell carcinoma ...