Bacterial tRNA adenosine deaminases (TadAs) catalyze the hydrolytic deamination of adenosine to inosine at the wobble position of tRNA(Arg2), a process that enables this single tRNA to recognize three different arginine codons in mRNA. In addition, inosine is also introduced at the wobble position...
An adenosine deaminase that generates inosine at the wobble position of tRNAs. Science 286, 1146–1149 (1999). CAS PubMed Google Scholar Torres, A. G. et al. Inosine modifications in human tRNAs are incorporated at the precursor tRNA level. Nucleic Acids Res. 43, 5145–5157 (2015). CAS...
N1-methyladenosine (m1A), mono-methylation of adenosine moiety in N1 position, is a highly conserved modification known to target tRNAs that promotes their stability and proper folding. Loss of m1A modification on tRNALysinduces the misfolding of tRNA, which thereby affects tRNALysfunction (Pan201...
coli tRNA adenosine deaminase (TadA) in a highly specific manner. In contrast, in yeast, seven cytoplasmic tRNAs contain I34, which are probably formed by a single Tad2p/Tad3p complex with lower substrate specificity. Thus, during the evolution from prokaryotes to eukaryotes, TilS may have ...
We have identified the Arabidopsis thaliana gene that codes for tRNA adenosine deaminase arginine (TADA), a chloroplast tRNA editing protein specifically required for deamination of chloroplast (cp)-tRNAArg(ACG) to cp-tRNAArg (ICG). Land plant TADAs have a C-terminal domain similar in sequence ...
36 Dzikowska A, Grzelak A, Gawlik J et al: KAEA (SUDPRO), a member of the ubiquitous KEOPS/EKC protein complex, regulates the arginine catabolic pathway and the expression of several other genes in Aspergillus nidulans. Gene 2015; 573: 310–320. 37 Mallari JP, Oksman A, Vaupel B, ...
Life without tRNAArg-adenosine deaminase TadA: Evolutionary consequences of decoding the four CGN codons as arginine in Mycoplasmas and other Mollicutes. Nucleic Acids Res. 2013, 41, 6531–6543. [CrossRef] [PubMed] 107. Torres, A.G.; Piñeyro, D.; Filonava, L.; Stracker, T.H.; ...
It was shown that the orthogonal tRNAoptAUG is a substrate for TadA, an A to I editing deaminase in E. coli that is usually involved in essential maturation steps of endogenous tRNAs [209]. Reverse transcription, amplification and sequencing experiments demonstrated that the adenosine at position...