The usage of the TRAV1-2 gene in MR1-restricted TCRs facilitates a consistent docking mode atop the MR1. This docking mode maintains the evolutionary conserved Tyr-95α from the CDR3α loop in a conserved location, so that it protrudes deeply into the A′-pocket of the MR1-Ag-binding...
TCR ab CDR3 sequences and gene names of D462-E4 are shown, right. (d) BEAS2B cell line infected with mycobacteria and tested for their ability to stimulate T-cell clones in the presence of blocking antibodies or isotype by IFN-g ELISPOT assays, D462-E4, D481-F12 (TRAV1-2 þ ...
Generation of an MR1-knockout cell line using CRISPR/Cas9 The reagents used to mutate the MR1 gene were derived from the toolkit described in ref.49. A codon-optimized synthetic Cas9 cDNA under the control of the cytomegalovirus promoter (Addgene plasmid #41815) was used in combination with...