TCR ab CDR3 sequences and gene names of D462-E4 are shown, right. (d) BEAS2B cell line infected with mycobacteria and tested for their ability to stimulate T-cell clones in the presence of blocking antibodies or isotype by IFN-g ELISPOT assays, D462-E4, D481-F12 (TRAV1-2 þ ...
Generation of an MR1-knockout cell line using CRISPR/Cas9 The reagents used to mutate the MR1 gene were derived from the toolkit described in ref.49. A codon-optimized synthetic Cas9 cDNA under the control of the cytomegalovirus promoter (Addgene plasmid #41815) was used in combination with...
Gene Expression ProfilingDrug Resistance, NeoplasmTo the editor:Peripheral T-cell lymphomas (PTCL) are uncommon and account for <10% of all non-Hodgkin lymphomas.[1][1] The most common category of PTCL is "not otherwise specified" (PTCL-NOS), reflecting the lack of specific parameters to ...