The main protein involved in the organisation of the Ter macrodomain is the DNA-binding protein MatP, which binds to matS sequences located in the Ter domain [9]. MatP is also involved in linking the Ter macrodomain to the Z-ring involved in septation [10]. Int. J. Mol. Sci. 2021,...
berghei trap gene (PBANKA_134980) coding sequence (CDS) has been replaced by the CDS of P. falciparum trap (PF3D7_1335900), we used a 2-step GIMO transfection protocol [31,32]. In the first step we deleted the P. berghei trap CDS and replaced it with the positive-negative ...
A series of ter-cassettes, each containing terB sequences (5 -AGTATGTTGTAACTAAAG-3 ) and 20 bp overlapping ends for subsequent seamless ligation with the ends of the PKOZ fragment through an originally developed method (Recombination Assembly; to be published elsewhere), were PCR-amplified ...