TransStart®FastPfu DNA Polymerase250 U×1500 U×1500 U×6 5×TransStart®FastPfu Buffer1.2 ml×11.2 ml ×21.2 ml ×12 2.5 mM dNTPs- / 500 μl×1- / 1 ml ×1- / 1 ml ×6 50 mM MgSO4200 μl×1400 μl ×11 ml ×1
TransStart® FastPfu PCR SuperMix is a ready-to-use mixture of TransStart® FastPfu DNA polymerase, dNTPs, and optimized buffer, featuring high amplification efficiency, fast amplification speed, high fidelity and high specificity. The SuperMix is provided at 2× concentration and can be used at...
FastPfuBuffer(含Mg 2+ ) 100mMTris-SO 4 (pH9.2),200mMKCl,50mM(NH4)2SO4,10mMMgSO4,10%Glycerol,其它成分。 ●另外配有50mMMgSO4。 PCR体系 (50μl反应体系) 优化的参数 (50μl反应体系) ParameterTargets≤10kbTargets≥10kbcDNATargets 100ngGenomicDNA200-500ngGenomicDNA1-2μlcDNAfromRTreaction ...
FastPfuFlyBuffer 10μl1× 2.5mMdNTPs 5μl0.25mM 1μl2.5units ddH 2 O to50μlNotapplicable TransStart ® FastPfuFlyDNAPolymerase ComponentAP231-01/11AP231-02/12AP231-03/13 TransStart ® FastPfuFlyDNAPolymerase250U×1500U×1500U×6 ...
• Double cation (K+, NH4+) buffer enhances the specificity and reduces primer-dimer formation. • Passive reference dyes are suitable for different qPCR instruments (adjust the difference in each tube due to the error from PCR sample loading). Kit Contents Component AQ***1 AQ***2 AQ*...
TransStart® FastPfu Fly DNA Polymerase 250 U×1 500 U×1 500 U×6 2×TransStart® FastPfu Fly Reaction Mix 1 ml×3 1.2 ml ×5 1.2 ml ×30 50 mM MgSO4 200 μl×1 400 μl ×1 1 ml ×1 6×DNA Loading Buffer 500 μl×1 1 ml ×1 1 ml ×2 PCR Stimulant 200 μl×1 ...
This product contains TransStart® FastPfu Fly DNA Polymerase, dNTPs and optimized reaction buffer at a concentration of 2×. It has the features of high amplification efficiency and fast amplification speed (≤ 5 kb fragments can achieve 12 kb/min extreme amplification, > 5 kb fragments can ac...
5×TransStartFastPfuBuffer(含Mg 2+ )100mMTris-HCl(pH8.8),50mMKCl,50mM(NH4)2SO4,10mMMgSO4,其它成分。 ●另外配有50mMMgSO4。 PCR体系 (50μl反应体系) ComponentsVolumeFinalConcentration TemplateDNA<0.5μgasrequired ForwardPrimer(10μM)1-2μl0.2–0.4μMeach ...