1× Phosphate buffer saline (PBS) Meilunbio Cat #: MA0015,Lot#: MA0015-Dec-03E14 Dithiothreitol (DTT) Sangon Cat #: DB0058, Lot#: F222BA0006 Sodium chloride Sinopharm Cat #: 10019318, Lot#: 20200915 TransStart Fastpfu DNA Polymerase Transgen Cat #: AP221, Lot#: N30507 dNTPs Novoprote...
PCR amplification was performed using the TransStart FastPfu FLY DNA Polymerase (AP231, civic bioscience) following instructions of the manufacturer with primers described in supplemental Table 4. Sanger sequencing of amplicons was performed on an ABI 3730xl DNA Analyzer (Applied Biosystems) at McGill...
The PCR reaction system was constituted of 4 μL of 5×TransStart FastPfu buffer, 2 μL of 2.5 mM dNTPs, 0.8 μL of forward primer (5 μM), 0.8 μL of reverse primer (5 μM), 0.4 μL of TransStart FastPfu DNA polymerase, 10 ng of template DNA, and ddH2O to make up a final...