Spatial organization of protein biosynthesis in the eukaryotic cell has been studied for more than fifty years, thus many facts have already been included in textbooks. According to the classical view, mRNA transcripts encoding secreted and transmembrane proteins are translated by ribosomes associated ...
Efficient modification and preparation of circular DNA for expression in cell culture Article Open access 21 December 2022 Introduction Circular RNAs (circRNAs) endogenous to eukaryotic cells have drawn increasing interest due to their prevalence and range of potential biological functions1. Most circRNAs...
Di R, Dinesh-Kumar SP, Miller WA: Translational frameshifting by barley yellow dwarf virus RNA (PAV serotype) inEscherichia coliand in eukaryotic cell-free extracts. Mol Plant-Microbe Interact 6: 444–452 (1993). PubMedCASGoogle Scholar Dinesh-Kumar SP, Brault V, Miller WA: Precise mapping ...
The stability of protein expression from circRNA is also dependent on circRNA purity and the sensitivity of transfected cell types to contaminating RNA species. Non-circular Components of the Splicing Reaction Contribute to Immunogenicity To explore the source of immunogenicity in circRNA splicing ...
In subject area: Biochemistry, Genetics and Molecular Biology The canonical model of eukaryotic translation initiation describes a process of assembly of the 48S ribosome complex at the 5′ untranslated region (5′-UTR) of an mRNA. From: Trends in Biotechnology, 2020 ...
Poliovirus RNA stimulates incorporation of35S from both [35S]methionine and formyl-[35S]methionyl-tRNAfMetin cell-free systems derived from HeLa cells or from poliovirus-infected HeLa cells. The largest product formed under the direction of the viral RNA is the same size as the polyprotein though...
Complex RNA structures in 5′ UTRs, such as RNA G-quadruplexes, may serve as steric blocks to RNA structure unwinding by the scanning ribosome, eukaryotic initiation factor 4A (eIF4A) and other helicases. Internal ribosome entry sites (IRESs) recruit ribosomes to 5′ UTRs in a cap-indepen...
During mammalian programmed cell death, cleavage of the translation initiation factor 4G proteins (eIF4GI and eIF4GII) by caspase-3 induces the cap-indepen... V Contreras,MA Richardson,E Hao,... - 《Cell Death & Differentiation》 被引量: 61发表: 2008年 Translation of eukaryotic mRNA in ...
Mammalian eukaryotic translation initiation factor 3 (eIF3) is the largest complex of the translation initiation factors. The eIF3 complex is comprised of thirteen subunits, which are named eIF3a to eIF3 m in most multicellular organisms. TheeIF3egene locus is one of the most frequent integration ...
Careful evaluation of such therapies is crucially needed considering the central role of eIF2α pathway in cellular function for every organ. 展开 关键词: diabetes endoplasmic reticulum eukaryotic translation initiation factor 2 protein phosphorylation protein synthesis therapeutic approaches β cell failure ...